microRNA expression profile in undifferentiated gastric cancer
microRNA expression profile in undifferentiated gastric cancer
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DOI：
10.3892/ijo_00000179
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发表时间：
2009-02-01
影响因子：
5.2
通讯作者：
Fujii, Yoshitaka
中科院分区：
文献类型：
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作者：
Katada, Takeyasu;Ishiguro, Hideyuki;Fujii, Yoshitaka
未分化胃癌患者的预后一般较差。各种microRNA（miRNAs）在未分化胃癌中的表达尚未得到全面研究。从42例未分化胃癌组织和配对正常胃组织中提取总RNA。对一组72个miRNA进行定量逆转录酶-聚合酶链反应（qRT-PCR）。与配对的正常组织相比，未分化胃癌组织中3种miRNA（mir-34 b、mir-34 c和mir-128 a）的表达水平显著上调，3种miRNA（mir-128 b、mir-129和mir-149）的表达水平下调。mir-20 b或150高表达水平的患者的生存概率显着较低。mir-27 a与淋巴结转移有关。我们的研究提供了一系列可能与未分化胃癌患者预后相关的候选miRNAs。这些miRNAs的靶基因及其功能有待进一步研究。
Prognosis of patients with undifferentiated gastric cancer is generally poor. The expression of various microRNAs (miRNAs) has not been comprehensively investigated in undifferentiated gastric cancer. Total RNA was extracted from the specimens of 42 undifferentiated gastric cancer tissues and paired normal gastric tissue. Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed for a set of 72 miRNAs. The expression of each miRNA relative to the internal control RNA was determined using the 2(Delta Ct) method, The expression levels of 3 miRNAs (mir-34b, mir-34c and mir-128a) were significantly upregulated and those of 3 miRNAs (mir-128b, mir-129 and mir-149) were downregulated in undifferentiated gastric cancer tissue when compared with those of the paired normal tissues. The probability of survival was significantly lower in patients with high expression levels of mir-20b or 150. There was a correlation between mir-27a and lymph node metastasis. Our investigation provides a list of candidate miRNAs that may be associated with the prognosis in undifferentiated gastric cancer patients. Further study is warranted to identify the target genes of these miRNAs and their function.