Harvesting human adipose tissue-derived adult stem cells: resection versus liposuction
Harvesting human adipose tissue-derived adult stem cells: resection versus liposuction
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DOI：
10.3109/14653240903204322
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发表时间：
2009-01-01
期刊：
影响因子：
4.5
通讯作者：
Prantl, Lukas
中科院分区：
文献类型：
--
作者：
Schreml, Stephan;Babilas, Philipp;Prantl, Lukas
背景脂肪组织是间充质干细胞的丰富来源，可用于组织工程。本研究的目的是从人脂肪组织来源的干细胞的活性、细胞数和分化潜能方面，确定更适合于获取人脂肪组织源干细胞的方法，即手术切除或吸脂。随后，对HASC进行培养、荧光激活细胞分选(FACS)分析，并在成脂、成骨和成软骨条件下进行分化。结果吸脂组和手术切除组的HASC细胞数差异无统计学意义(P=0.086)。吸脂组活细胞百分率显著高于切除组(P=0.002)。成脂分化潜能差异无统计学意义(P=0.179)。从吸脂材料获得的培养物数量明显少于从切除材料获得的可分化为骨细胞(P=0.049)和软骨细胞(P=0.012)的数量。讨论尽管来自脂肪抽吸材料的某些细胞系不能像手术切除材料那样频繁地分化，但对于某些组织工程目的，吸脂可能是更好的，尤其是因为更具侵入性的收获过程，更高的细胞存活率，以及在成脂分化潜能方面，脂肪抽吸术和切除的HASC之间没有显著差异。
Background Adipose tissue is an abundant source of mesenchymal stem cells (MSC), which can be used for tissue-engineering purposes. The aim of our study was to determine the more suitable procedure, surgical resection or liposuction, for harvesting human adipose tissue-derived stem cells (hASC) with regard to viability, cell count and differentiation potential.Methods After harvesting hASC, trypan blue staining and cell counting were carried out. Subsequently, hASC were cultured, analyzed by fluorescence-activated cell sorting (FACS) and differentiated under adipogenic, osteogenic and chondrogenic conditions. Histologic and functional analyzes were performed at the end of the differentiation period.Results No significant difference was found with regard to the cell counts of hASC from liposuction and surgically resected material (P = 0.086). The percentage of viable cells was significantly higher for liposuction aspirates than for resection material (P = 0.002). No significant difference was found in the adipogenic differentiation potential (P = 0.179). A significantly lower number of cultures obtained from liposuction material than from resection material could be differentiated into osteocytes (P = 0.049) and chondrocytes (P = 0.012).Discussion Even though some lineages from lipoaspirated hASC can not be differentiated as frequently as those from surgically resected material, liposuction may be superior for some tissue-engineering purposes, particularly because of the less invasive harvesting procedure, the higher percentage of viable cells and the fact that there is no significant difference between lipoaspirated and resected hASC with regard to adipogenic differentiation potential.