Purification and characterization of a novel cypermethrin-hydrolyzing esterase from Bacillus licheniformis B-1
Purification and characterization of a novel cypermethrin-hydrolyzing esterase from Bacillus licheniformis B-1
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地衣芽孢杆菌 B-1 中新型氯氰菊酯水解酯酶的纯化和表征
DOI：
10.1111/1750-3841.15662
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发表时间：
2021
影响因子：
3.9
通讯作者：
Shuliang Liu
中科院分区：
文献类型：
--
作者：
Mengmei Zhang;Wen Lai;Yuanting Zhu;Shujuan Chen;Kang Zhou;Xiaolin Ao;Li He;Yong Yang;Likou Zou;Aiping Liu;Kai Yao;Shuliang Liu
AbstractCypermethrin (CY) is a synthetic pyrethroid widely used to control insect pests and it elicits a toxic effect on the human body. In this study,Bacillus licheniformisB‐1 isolated from tea garden soil was used to degrade CY effectively. A specific enzyme was mainly localized in the extracellular compartments of B‐1. This enzyme was identified as an esterase that could be produced without CY. The enzyme was purified 23.03‐fold to apparent homogeneity with 8.38% overall recovery by ammonium sulfate precipitation, anion exchange chromatography, and gel filtration chromatography. The molecular mass of the CY‐degrading enzyme was 66.4 kDa, and its optimal pH and temperature were 8.5 and 40 °C, respectively. Appropriate Zn2+, Mn2+, Mg2+, Tween 80, SDS, Triton X‐100, and BSA concentrations could greatly increase the activity of this enzyme. By contrast, EDTA, 1,10‐phenanthroline, NaF, and PMSF strongly inhibited its activity. The purified enzyme showedKmandVmaxvalues were 5.532 nmol/mL and 33.445 nmol/min. The CY residue in lettuce and cherry tomatoes could be removed more than 50% under the conditions of the treatment concentration for 500 mg/L and the enzyme preparation dilution of 100 times. These results suggested that the CY‐degrading enzyme, a constitutive enzyme that mainly exists in the extracellular space, was a novel esterase that might be used to detoxify CY, and could remove CY in vegetables effectively.Practical ApplicationOur research found a novel cypermethrin‐hydrolyzing esterase fromBacillus licheniformisB‐1 and proved that the enzyme could remove cypermethrin in vegetables effectively.
