Ginsenoside Rg1 ameliorates testicular senescence changes in D‑gal‑induced aging mice via anti‑inflammatory and antioxidative mechanisms.
Ginsenoside Rg1 ameliorates testicular senescence changes in D‑gal‑induced aging mice via anti‑inflammatory and antioxidative mechanisms.
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人参皂苷 Rg1 通过抗炎和抗氧化机制改善 D-gal 诱导的衰老小鼠睾丸衰老变化
DOI：
10.3892/mmr.2018.8659
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发表时间：
2018-05
影响因子：
3.4
通讯作者：
Wang YP
中科院分区：
文献类型：
--
作者：
Wang ZL;Chen LB;Qiu Z;Chen XB;Liu Y;Li J;Wang L;Wang YP
随着人口增长、老龄化加剧，寿命延长以及抗衰老已成为生命科学和社会科学的热门研究领域。随着年龄增长，男性生殖系统中产生精子和雄激素的器官——睾丸的结构和功能逐渐衰退。人参皂苷Rg1是中药人参的一种提取物。该提取物通过其抗炎和抗氧化特性起到抗衰老作用。然而，人参皂苷Rg1是否能延缓睾丸衰老尚未见报道。本研究建立了D - 半乳糖（D - gal）诱导的衰老小鼠模型，以研究人参皂苷Rg1对睾丸结构和功能的保护作用及其潜在机制。将60只健康的无特定病原体雄性C57BL / 6小鼠随机分为四组：对照组；Rg1组；D - gal + Rg1组；D - gal组。小鼠组织用于进一步实验。本研究进一步研究了Rg1对D - gal诱导的衰老小鼠血清睾酮含量、睾丸指数、睾丸微观结构、生精细胞衰老、生精细胞凋亡、抗氧化酶活性、炎症因子水平以及S期激酶相关蛋白（p19）、细胞周期蛋白依赖性激酶抑制剂1（p21）和细胞肿瘤抗原p53（p53）水平的影响。总体而言，与D - gal组相比，Rg1处理提高了睾丸指数、血清睾酮水平以及超氧化物歧化酶活性含量和总抗氧化能力。睾丸组织中衰老相关β - 半乳糖苷酶阳性细胞百分比、细胞凋亡水平以及丙二醛、肿瘤坏死因子 - α、白细胞介素（IL）- 1β和IL - 6含量显著降低，并且由于Rg1处理，p19、p53和p21的表达下调。本研究结果表明，人参皂苷Rg1能够保护小鼠睾丸免受D - gal诱导的衰老。此外，Rg1的保护作用可能通过抗氧化以及下调p19 / p53 / p21信号通路来实现。
With the growing population, aging, extended lifespans and anti-aging have become popular areas of research in the life and social sciences. With increasing age, the structure and function of the testes, the spermatogenetic and androgen-producing organ in the male reproductive system, gradually declines. Ginsenoside Rg1 is an extract of Panax ginseng in traditional Chinese medicine. The extract facilitates anti-aging through its anti-inflammatory and antioxidant properties. However, it has not been reported whether ginsenoside Rg1 delays testicular aging. The present study established D-galactose (D-gal)-induced aging mouse models to examine the protective effects of ginsenoside Rg1 on the structure and function of the testes, and the underlying mechanism. A total of 60 healthy specific pathogen-free male C57BL/6 mice were randomly divided into four groups: Control group; Rg1 group; D-gal + Rg1 group; and D-gal group. The tissues of the mice were used for further experiments. The present study further investigated the effects of Rg1 on the volume of serum testosterone, the testicular index, testicular microscopic structures, the senescence of spermatogenetic cells, the apoptosis of spermatogenetic cells, the activity of the antioxidant enzymes, the levels of inflammatory cytokines, and the levels of S-phase kinase-associated protein (p19), cyclin-dependent kinase inhibitor 1 (p21) and cellular tumor antigen p53 (p53) in D-gal-induced aging mice. In general, compared with the D-gal group, the treatment of Rg1 increased the testis index, serum testosterone level and the active content of superoxide dismutase and the total antioxidant capacity. The percentage of senescence-associated β-galactosidase-positive cells, the level of apoptosis and the volume of methane dicarboxylic aldehyde, tumor necrosis factor-α, interleukin (IL)-1β and IL-6 in testicular tissues were significantly decreased, and the expression of p19, p53 and p21 was downregulated due to the treatment with Rg1. The results of the present study demonstrated that ginsenoside Rg1 was able to protect the testes against D-gal-induced aging in mice. In addition, the protective effect of Rg1 may be achieved via antioxidation and downregulation of the p19/p53/p21 signaling pathway.
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影响因子：
25
作者：
Russo, Scott J.;Murroughl, James W.;Han, Ming-Hu;Charney, Dennis S.;Nestler, Eric J.
通讯作者：
Nestler, Eric J.
影响因子：
4.5
作者：
Van Raamsdonk, Jeremy M.;Hekimi, Siegfried
通讯作者：
Hekimi, Siegfried
DOI：
10.1083/jcb.201009094
发表时间：
2011-02-21
期刊：
The Journal of cell biology
影响因子：
--
作者：
Rodier F;Campisi J
通讯作者：
Campisi J
影响因子：
7.8
作者：
Gambino V;De Michele G;Venezia O;Migliaccio P;Dall'Olio V;Bernard L;Minardi SP;Della Fazia MA;Bartoli D;Servillo G;Alcalay M;Luzi L;Giorgio M;Scrable H;Pelicci PG;Migliaccio E
通讯作者：
Migliaccio E
影响因子：
5.8
作者：
GRAY, A;FELDMAN, HA;LONGCOPE, C
通讯作者：
LONGCOPE, C