Determination of kinetic changes of aggrecan-hyaluronan interactions in solution from its rheological properties.
Determination of kinetic changes of aggrecan-hyaluronan interactions in solution from its rheological properties.
复制标题
根据其流变特性测定溶液中聚集蛋白聚糖-透明质酸相互作用的动力学变化。
DOI：
10.1016/0021-9290(94)90066-3
复制
发表时间：
1994
影响因子：
2.4
通讯作者：
Tang,LH
中科院分区：
文献类型：
--
作者：
Zhu,W;Mow,VC;Rosenberg,LC;Tang,LH
利用锥板粘度计研究了聚集的软骨蛋白聚糖（aggrecan）和透明质酸之间的相互作用动力学。两种类型的分子的混合直接在粘度计的板上进行，并且聚集过程通过样品的稳态剪切粘度和/或动态剪切模量作为时间的函数的变化来监测。流动条件对聚集蛋白聚糖-透明质酸相互作用速率的影响通过使样品在指定剪切速率下经受稳定剪切运动以及在指定频率和振幅下经受振荡剪切运动来检查。聚集蛋白聚糖和透明质酸分子之间的相互作用的动力学特性不仅取决于蛋白聚糖聚集发生的流动条件，而且取决于溶液中组分的浓度。在高剪切速率（> 10 s-1）下，混合物溶液的粘度单调增加，开始接近聚集蛋白聚糖溶液的粘度，并在约35 min内达到聚集体溶液的粘度。令人惊讶的是，在慢剪切运动（< 10 s-1）下，混合物溶液的粘度超过相同透明质酸：聚集蛋白聚糖比率和浓度下的对照聚集体溶液的粘度。此外，振荡运动下的聚集发生在接近生理频率（10 rad s-1），尽管聚集过程的速率比稳态剪切运动（> 100 min）下慢得多。然而，高频振荡剪切（62.8 rad s-1）往往会阻碍聚集，导致动态模量随时间推移而降低。在这项研究中观察到的聚集率和聚集体大小的负载条件的影响似乎表明蛋白多糖的结构和含量之间的密切关系，和整个关节的生理应力和频率的关节运动。
The kinetics of interactions between aggregating cartilage proteoglycan (aggrecan) and hyaluronan was examined through their rheological flow behavior using a cone on plate viscometer. The mixing of the two types of molecules was carried out directly on the plate of the viscometer, and aggregation process was monitored through the changes of the sample's steady-shear viscosity and/or dynamic shear modulus as a function of time. The effect of flow conditions on the aggrecan-hyaluronan interaction rates was examined by subjecting samples to steady-shearing motions at specified shear rates, and to oscillatory shear motions of specified frequencies and amplitudes. The characteristics of the kinetics of interaction between aggrecan and hyaluronan molecules depended not only on the flow conditions under which proteoglycan aggregation took place, but also on the concentration of the components in the solution. At high shear rates (> 10 s−1), viscosity of the mixture solution increased monotonically, starting near the viscosity of the aggrecan solution, and reaching the viscosity of the aggregate solution in approximately 35 min. Surprisingly, under slow shearing motions (< 10 s−1), the viscosities of the mixture solutions exceeded those of control aggregate solutions at identical hyaluronan: aggrecan ratios and concentrations. In addition, the aggregation under oscillatory motions took place near physiologic frequency (10 rad s−1) although the rate of aggregation process was much slower than under steady-shearing motion (> 100 min). However, the high-frequency oscillatory shearing (62.8 rad s−1) tended to impede aggregation resulting in a reduction of dynamic modulus over time. The influence of loading conditions on the rate of aggregation and aggregate size observed in this study seems to suggest a close relationship between proteoglycan structure and content, and degree of physiological stress throughout the joint and frequency of the joint motion.