RIBOFLAVIN DEFICIENCY, METABOLIC-RATE AND BROWN ADIPOSE-TISSUE FUNCTION IN SUCKING AND WEANLING RATS
RIBOFLAVIN DEFICIENCY, METABOLIC-RATE AND BROWN ADIPOSE-TISSUE FUNCTION IN SUCKING AND WEANLING RATS
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DOI：
10.1079/bjn19890137
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发表时间：
1989-05-01
影响因子：
3.6
通讯作者：
BATES, CJ
中科院分区：
文献类型：
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作者：
PATTERSON, BE;BATES, CJ
核黄素缺乏对生长的影响，全身耗氧量，细胞色素c氧化酶（EC 1.9.3.1）的活性和棕色脂肪组织的GDP结合能力进行了测量，在三组大鼠：哺乳幼崽，断奶大鼠，和母鼠。对照组为体重匹配、成对喂养或随意喂养。正如预测的那样，核黄素缺乏降低了生长速度，增加了红细胞谷胱甘肽还原酶（NAD（P）H）的活化系数（EC 1.6.4.2）。在吸吮幼崽，它也减少了全身O2消耗每单位体重，特别是去甲肾上腺素刺激后。然而，在断乳大鼠中，去甲肾上腺素刺激前后O2消耗量均增加。棕色脂肪组织的细胞色素c氧化酶（EC 1.9.9.1）活性并不总是受到核黄素缺乏的影响。[3H]GDP与线粒体的结合在缺乏断奶大鼠中增加。因此，断奶大鼠似乎更能承受严重消耗的影响。它们的生长减缓和非颤抖性产热增加有助于抵消核黄素与其他组织构建材料的不利比例。核黄素缺乏的儿童体温调节的相关性进行了讨论。
The effects of riboflavin deficiency on growth, whole-body oxygen consumption, cytochrome c oxidase (EC 1.9.3.1) activity and GDP-binding capacity of brown adipose tissue were measured in three groups of rats: sucking pups, weanling rats, and dams. Control groups were weight-matched, pair-fed or fed ad lib. Riboflavin deficiency reduced growth rate and increased the activation coefficient of erythrocyte glutathione reductase (NAD(P)H) (EC 1.6.4.2), as predicted. In sucking pups it also reduced whole-body O2 consumption per unit body-weight, especially after noradrenaline stimulation. In weanling rats, however, it increased O2 consumption both before and after noradrenaline stimulation. Cytochrome c oxidase (EC 1.9.9.1) activity of brown adipose tissue was not consistently affected by riboflavin deficiency. Binding of [3H]GDP to the mitochondria was increased in the deficient weanling rats. Weanling rats therefore, seemed better able to withstand the effects of severe depletion. Their reduced growth and increased non-shivering thermogenesis helped to counteract the unfavorable ratio of riboflavin: other tissue-building materials. The relevance for thermoregulation in riboflavin-deficient children is discussed.