IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans
IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans
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DOI：
10.1136/jcp.33.1.98-b
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发表时间：
1980-01
影响因子：
3.4
通讯作者：
R. Carter
中科院分区：
文献类型：
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作者：
R. Carter
用于检测免疫球蛋白的免疫过氧化物酶方法是一个冗长的程序，并且通常涉及胰蛋白酶孵育阶段。根据我们的经验，切片，特别是皮肤活检的切片，经常会隆起或完全消失。已经尝试了诸如白蛋白、Chrome明矾和支链淀粉之类的粘合剂，但没有成功，并且Romac C331不再可用。然而，当用少量石油醚稀释、涂抹在载玻片上并使其干燥时，橡胶溶液（Li-Lo Ltd，Woking，Surrev，但也可从大多数文具店获得）Cow Gum是完全有效的粘合剂。然后将切片漂浮在制备的载玻片上，并在37 ℃下孵育过夜。该粘合剂是透明的，不影响免疫过氧化物酶反应，并且在二甲苯中不溶，以便将切片带到水中，最后以通常的方式复染。牛胶涂片的非粘性表面是一个优点，因为它通过增加接触角限制了试剂的扩散，从而可以使用更小的体积，并降低切片干燥的风险。
The immunoperoxidase method for the detection of immunoglobulins is a lengthy procedure and often involves a trypsin incubation stage. In our experience, sections-particularly from skin biopsies-often lift or are lost altogether. Adhesives such as albumin, chrome alum, and amylopectin have been tried without success, and Romac C331 is no longer available. However, Cow Gum, a rubber solution (Li-Lo Ltd, Woking, Surrev, but also available from most stationers) when diluted with a little petroleum ether, smeared onto slides, and allowed to dry is a completely effective adhesive. Sections are then floated onto the prepared slides and inicubated at 37TC overnight. The adhesive is clear, does not affect immunoperoxidase reactions, andis sufficiently insolublein xylene for the sections to be brought to water and finally counterstained in the usual way. The non-wettable surface of the Cow Gum smear is an advantage in that it limits spreading of thereagents by increasing the angle of contact so that smaller volumes may be used and the risk of the section drying out is reduced.