Topographic distribution of homing receptors on B and T cells in human gut-associated lymphoid tissue: relation of L-selectin and integrin alpha 4 beta 7 to naive and memory phenotypes.
Topographic distribution of homing receptors on B and T cells in human gut-associated lymphoid tissue: relation of L-selectin and integrin alpha 4 beta 7 to naive and memory phenotypes.
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人肠道相关淋巴组织中 B 细胞和 T 细胞上归巢受体的拓扑分布：L-选择素和整合素 α 4 β 7 与幼稚和记忆表型的关系。
DOI：
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发表时间：
1997
影响因子：
6
通讯作者：
P. Brandtzaeg
中科院分区：
文献类型：
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作者：
I. Farstad;T. Halstensen;D. Kvale;O. Fausa;P. Brandtzaeg
在小鼠中，整合素α 4 β 7是淋巴细胞使用的主要受体，淋巴细胞归巢到派尔集合淋巴结，尽管L-选择素有助于与高内皮微静脉的初始相互作用。对这些粘附分子在人体中的表达和功能知之甚少。在人派伊尔集合淋巴结（n = 8）和阑尾（n = 4）（统称为肠道相关淋巴组织）中检查了L-选择素和α 4 β 7在各种B-和T-细胞亚群上的分布。在冰冻切片上进行多色免疫分型，并通过流式细胞术检查分散的细胞。在冷冻切片中，滤泡间高内皮微静脉周围和内部的CD 45 RA + T细胞以及滤泡外套膜中的表面IgD+ B淋巴细胞通常表达丰富的L-选择素，但仅表达中等水平的α 4 β 7。CD 45 RO + T细胞和sIgD- B细胞表达更高水平的α 4 β 7，并且通常位于推定的传出神经系统附近;只有一小部分（< 20%）的这种记忆细胞表达L-选择素。通过流式细胞术，显著多于B淋巴细胞共表达L-选择素和α 4 β 7（分别为40%对25%和67%对39%）。在具有许多L-选择素+细胞（> 30%）的样品中，这些淋巴细胞中的更多的共表达α 4 β 7，而不是在具有很少L-选择素+细胞的样品中。因为L-选择素和α 4 β 7在位于高内皮微静脉附近的淋巴细胞上共表达，并且因为当存在许多L-选择素+细胞时，这种共表达相对常见，所以这两种分子可能参与归巢至人肠道相关淋巴组织。这种归巢可能在T淋巴细胞中最明显，发现T淋巴细胞比B淋巴细胞更常表达L-选择素和α 4 β 7。位于传出神经系统附近的记忆细胞上α 4 β 7的选择性和相对较高的表达表明了不同的迁移能力;从肠道相关淋巴组织退出后，这种受刺激的细胞可能主要回到粘膜效应位点。
In mice, integrin alpha 4 beta 7 is the main receptor used by lymphocytes that home to the Peyer's patches, although L-selectin contributes to the initial interaction with high endothelial venules. Less is known about the expression and function of these adhesion molecules in humans. The distribution of L-selectin and alpha 4 beta 7 on various B- and T-cell subsets was examined in human Peyer's patches (n = 8) and appendix (n = 4), collectively called gut-associated lymphoid tissue. Multicolor immunophenotyping was performed on cryosections, and dispersed cells were examined by flow cytometry. In cryosections, CD45RA+ T cells around and within interfollicular high endothelial venules, as well as surface (s)IgD+ B lymphocytes in the follicle mantles, often expressed abundant L-selectin but only intermediate levels of alpha 4 beta 7. CD45RO+ T cells and sIgD- B cells expressed higher levels of alpha 4 beta 7 and were often located near putative efferent lymphatics; only a small fraction (< 20%) of such memory cells expressed L-selectin. By flow cytometry, considerably more T than B lymphocytes co-expressed L-selectin and alpha 4 beta 7 (40% versus 25% and 67% versus 39%, respectively). In samples with many L-selectin+ cells (> 30%), more of these lymphocytes co-expressed alpha 4 beta 7 than in samples with few L-selectin+ cells. Because L-selectin and alpha 4 beta 7 were co-expressed on lymphocytes located near high endothelial venules, and because such co-expression was relatively common when many L-selectin+ cells were present, both of these molecules might participate in homing to human gut-associated lymphoid tissue. Such homing is probably most pronounced for T lymphocytes that were found to express L-selectin and alpha 4 beta 7 more often than B lymphocytes. The selective and relatively high expression of alpha 4 beta 7 on memory cells located near efferent lymphatics indicated a different migratory capacity; after exit from gut-associated lymphoid tissue, such stimulated cells might home mainly to mucosal effector sites.