Visualization of GFP-expressing tumors and metastasis in vivo
Visualization of GFP-expressing tumors and metastasis in vivo
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DOI：
10.2144/01305bi01
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发表时间：
2001-05-01
期刊：
影响因子：
2.7
通讯作者：
Hoffman, RM
中科院分区：
文献类型：
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作者：
Hoffman, RM
我们已经开发了转移性癌症的小鼠模型，具有遗传荧光肿瘤，可以在新鲜组织中原位以及外部成像。为了实现这一能力，我们已经转导的绿色荧光蛋白（GFP）基因，克隆自生物发光水母Aequorea维多利亚，到一系列的人类和啮齿类动物的癌细胞系，在体外选择稳定表达GFP在体内移植肿瘤后，GFP在体内。利用这种荧光工具，我们首次在新鲜活组织或宿主器官原位检测和可视化肿瘤和转移，直至单细胞水平。结肠、前列腺、乳腺、脑、肝、淋巴结、肺、胰腺、骨和其他器官上的GFP肿瘤也可以通过定量全身荧光光学成像在外部、透射地可视化。实时肿瘤和转移性生长和血管生成以及代表性药物的抑制可以被成像和定量，用于快速抗肿瘤、抗转移和抗血管生成药物筛选。GFP转染的肿瘤细胞使体内肿瘤生长和转移的真实的时间可视化有了根本性的进展。
We have developed mouse models of metastatic cancer with genetically fluorescent tumors that can be imaged in fresh tissue, in situ, as well as externally. To achieve this capability, we have transduced the green fluorescent protein (GFP) gene, cloned from the bioluminescent jellyfish Aequorea victoria, into a series of human and rodent cancer cell lines that were selected in vitro to stably express GFP in vivo after transplantation of tumors by GFP in vivo. With this fluorescent tool, we detected and visualized for the first time tumors and metastasis in fresh viable tissue or in situ in host organs down to the single-cell level. GFP tumors on the colon, prostate, breast, brain, liver, lymph nodes, lungs, pancreas, bone, and other organs can also be visualized externally, transcutaneously by quantitative whole-body fluorescence optical imaging. Real-time tumor and metastatic growth and angiogenesis and inhibition by representative drugs can be imaged and quantified for rapid antitumor, antimetastatic, and antiangiogenesis drug screening. The GFP-transfected tumor cells enabled a fundamental advance in the visualization of tumor growth and metastasis in real time in vivo.