Evolutionary analysis of CBL-interacting protein kinase gene family in plants
Evolutionary analysis of CBL-interacting protein kinase gene family in plants
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DOI：
10.1007/s10725-013-9808-5
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发表时间：
2013-03
影响因子：
4.2
通讯作者：
C. Ye;X. Xia;W. Yin
中科院分区：
文献类型：
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作者：
C. Ye;X. Xia;W. Yin
钙调磷酸酶b样蛋白（CBLs）和钙调磷酸酶相互作用的蛋白激酶（CIPKs）形成CBL-CIPK复合物，在植物体内感知钙信号并将信号传递给下游反应。为了进一步了解CBL-CIPK信号系统，我们将重点研究cipk的进化分析。我们重新评估了8个植物基因组，鉴定出146个CIPKs，为水稻和杨树提供了几个新的CIPKs。构建了系统发育树，将这146个cipk分为富含内含子和不含内含子的进化枝。此外，所有来自非被子植物物种的cipk都存在于富含内含子的进化枝中。我们在这146个cipk中鉴定出30个保守的蛋白基序。基因重复分析表明，cipk在两个进化支中的扩增部分是由片段重复引起的，而串联重复仅在无内含子进化支中发现。Ka/Ks比值表明cipk基因经历了净化选择压力。此外，基因表达的聚类分析显示，在非生物胁迫下，两个支系中有一些cipk基因具有相似的表达模式，而四个cipksin内含子缺失支系形成了不同的表达模式，表明cipk基因在非生物胁迫下表达的复杂性。综上所述，我们的研究结果为CIPKs的进化提供了一些新的见解，并提示CIPKs无内含子分支的扩展是适应环境胁迫的。
Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) form the CBL-CIPK complexes, perceiving calcium signals and relaying the signals to downstream responses in plants. To further understand the CBL-CIPK signaling system, here we focused on the evolutionary analysis of CIPKs. We re-evaluated eight plant genomes and identified 146 CIPKs, providing several new CIPKs in rice and poplar. A phylogenetic tree was constructed, showing that these 146 CIPKs are grouped into intron-rich and intron-less clades. Furthermore, all the CIPKs from the non-angiosperm species were found in intron-rich clade. We identified 30 conserved protein motifs among these 146 CIPKs. Analysis of gene duplication showed that the expansion ofCIPKsin both clades is partly contributed by segmental duplications, however, tandem duplicates were found only in intron-less clade. Ka/Ks ratios showed thatCIPKgenes have experienced purifying selective pressure. Additionally, clustering of gene expression revealed that someCIPKgenes in two clades share similar expression patterns under abiotic stresses and fourCIPKsin intron-less clade form a distinct cluster (i.e., different expression patterns), suggesting the complexity ofCIPKgene expression under abiotic stresses. Taken together, our results provided some new insights into the evolution of CIPKs and the hint that the expansion ofCIPKsin intron-less clade is adaptive to environmental stresses.