Eukaryotic translation initiation factor 5A is involved in pathogen-induced cell death and development of disease symptoms in Arabidopsis
Eukaryotic translation initiation factor 5A is involved in pathogen-induced cell death and development of disease symptoms in Arabidopsis
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DOI：
10.1104/pp.108.118869
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发表时间：
2008-09-01
期刊：
影响因子：
7.4
通讯作者：
Thompson, John E.
中科院分区：
文献类型：
--
作者：
Hopkins, Marianne T.;Lampi, Yulia;Thompson, John E.
真核细胞翻译起始因子5A(EIF5A)是一种高度保守的蛋白质，广泛存在于所有真核生物中。这项研究表明，植物eIF5A参与了一种常见的坏死性细菌植物病原菌诱导的疾病症状的发生。具体地说，AteIF5A-2是拟南芥中三个eIF5A基因之一，它被证明可以调节由致病力较强的丁香假单胞菌番茄DC3000(PST DC3000)感染引起的程序性细胞死亡。转AteIF5A-2基因拟南芥植株对PST DC3000诱导的细胞程序性死亡表现出明显的抗性，病原菌的生长和植株组织中的病害症状的发展也相应减少。AteIF5A-2的结构性过表达规避了野生型植物对AteIF5A-2蛋白表达的明显转录后调控，但并没有增加对PST DC3000毒力入侵的敏感性。然而，结构性过表达AteIF5A-2的转基因植株确实表现出与早熟细胞死亡一致的表型。结果表明，AteIF5A-2是导致植物细胞程序性死亡的信号转导途径中的关键元件。
Eukaryotic translation initiation factor 5A (eIF5A) is a highly conserved protein found in all eukaryotic kingdoms. This study demonstrates that plant eIF5A is involved in the development of disease symptoms induced by a common necrotrophic bacterial phytopathogen. Specifically, AteIF5A-2, one of the three eIF5A genes in Arabidopsis (Arabidopsis thaliana), is shown to regulate programmed cell death caused by infection with virulent Pseudomonas syringae pv tomato DC3000 (Pst DC3000). Transgenic Arabidopsis plants with constitutively suppressed AteIF5A-2 exhibited marked resistance to programmed cell death induced by virulent Pst DC3000, and there was a corresponding reduction in pathogen growth and development of disease symptoms in the plant tissue. Constitutive overexpression of AteIF5A-2 circumvented the apparent posttranscriptional regulation of AteIF5A-2 protein expression characteristic of wild-type plants but did not increase susceptibility to virulent Pst DC3000 ingression. The transgenic plants with constitutive AteIF5A-2 overexpression did, however, display phenotypes consistent with precocious cell death. The results indicate that AteIF5A-2 is a key element of the signal transduction pathway resulting in plant programmed cell death.