Overexpression of NIMA-related kinase 2 is associated with progression and poor prognosis of prostate cancer.
Overexpression of NIMA-related kinase 2 is associated with progression and poor prognosis of prostate cancer.
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NIMA相关激酶2的过度表达与前列腺癌的进展和不良预后相关
DOI：
10.1186/s12894-015-0085-7
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发表时间：
2015-08-29
期刊：
影响因子：
2
通讯作者：
Zhong WD
中科院分区：
文献类型：
--
作者：
Zeng YR;Han ZD;Wang C;Cai C;Huang YQ;Luo HW;Liu ZZ;Zhuo YJ;Dai QS;Zhao HB;Liang YX;Zhong WD
NEK2是一种丝氨酸/苏氨酸激酶，参与有丝分裂过程中中心体复制和纺锤体组装的调控。这些过程的失调导致染色体不稳定和非整倍体，这是许多实体瘤的标志性变化。然而，NEK2的异常表达是否与前列腺癌（PCa）患者的预后相关仍有待确定。采用定量RT-PCR方法检测NEK2在人前列腺癌细胞和原代前列腺癌组织中的表达。人PCa细胞中NEK2的表达被siRNA破坏。在体外细胞培养和体内异种移植物皮下植入的实验中，评估了消耗对细胞增殖、存活和致瘤性的影响。对在线Taylor数据集进行了计算机分析，以确定NEK2的表达水平是否与前列腺癌的临床病理特征相关。与良性人前列腺上皮细胞和组织相比，NEK2在人前列腺癌细胞和原发性前列腺癌组织中的表达升高。耗尽NEK2表达抑制人PCa细胞体外增殖和体内异种移植物生长。NEK2在PCa中的表达水平与Gleason评分及患者病理分期呈正相关。结果表明，NEK2的过表达有可能作为前列腺癌预后的生物标志物。进一步验证大样本池是有必要的。
The NIMA-related kinase 2 (NEK2) is a serine/threonine kinase that is involved in regulation of centrosome duplication and spindle assembly during mitosis. Dysregulation of these processes causes chromosome instability and aneuploidy, which are hallmark changes of many solid tumors. However, whether aberrant expression of NEK2 is associated with outcome of prostate cancer (PCa) patients remains to be determined. Expression of NEK2 in human PCa cells and primary PCa tissues was assessed by quantitative RT-PCR. Expression of NEK2 in human PCa cells was depleted with siRNA. Effects of the depletion on cell proliferation, survival, and tumorigenicity were assessed both in vitro with cell cultures and in vivo with subcutaneous implantation of xenografts. In silico analyses of the online Taylor dataset were carried out to determine whether the expression level of NEK2 correlated with the clinicopathological characteristics of prostate cancer. Compared with benign human prostatic epithelial cells and tissues, the expression of NEK2 was elevated in human PCa cells and primary PCa tissues. Depleting NEK2 expression inhibited human PCa cell proliferation in vitro and xenograft growth in vivo. Expression level of NEK2 in PCa positively correlated with the Gleason score and pathologic stage of the patient. The results suggest that overexpression of NEK2 has the potential to serve as a biomarker for PCa prognosis. Further validation with large sample pool is warrant.