Zebrafish heparin-binding neurotrophic factor enhances neurite outgrowth during its development
Zebrafish heparin-binding neurotrophic factor enhances neurite outgrowth during its development
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DOI：
10.1016/j.bbrc.2004.06.172
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发表时间：
2004-08-20
影响因子：
3.1
通讯作者：
Chou, CM
中科院分区：
文献类型：
--
作者：
Chang, MH;Huang, CJ;Chou, CM
肝素结合神经营养因子（HBNF）是一种分泌型肝素结合蛋白，含有高碱性和富含半胱氨酸的氨基酸残基。本研究克隆了斑马鱼HBNF的全长cDNA，并通过生物信息学分析确定了其基因组结构。斑马鱼HBNF基因由5个外显子和4个内含子组成，全长约82 kb。RT-PCR分析表明，斑马鱼HBNF转录本在成体脑和肠组织中表达量较高，而在其它组织中表达量较低。在胚胎发育过程中，斑马鱼HBNF转录本在胚胎发育早期适度表达，并逐渐下降。孵化后观察到较高的表达水平，并保持该水平至成年。斑马鱼HBNF的整体氨基酸序列显示与人HBNF的60%同一性，但与其他中期因子蛋白的同一性约为40%。与哺乳动物同源物一样，斑马鱼HBNF在没有NGF刺激的情况下可以诱导PC 12细胞显著的神经突起生长。此外，斑马鱼HBNF能够在胚胎发生过程中增强斑马鱼胚胎中广泛的神经突生长。总之，在斑马鱼中建立了一种可行的神经突生长的体内测定法。(C)2004爱思唯尔公司All rights reserved.
Heparin-binding neurotrophic factor (HBNF) is a secreted heparin-binding protein containing highly basic and cysteine-rich amino acid residues. In this study, we cloned the full-length HBNF cDNA from zebrafish and determined its genomic structure by bioinformatics analysis. Zebrafish HBNF gene is composed of five exons and four introns spanning approximately 82 kb. RTPCR analysis revealed that zebrafish HBNF transcript was highly expressed in adult brain and intestine tissues while less in other tissues. During embryogenesis, zebrafish HBNF transcript was observed to be moderately expressed at earlier stages with a gradual decline. Higher expression level was observed after hatching and maintaining this level into adulthood. The overall amino acid sequence of zebrafish HBNF shows 60% identity to human HBNF, but with approximately 40% identity to other midkine proteins. Like mammalian homolog, zebrafish HBNF could induce significant neurite outgrowth in PC12 cells without NGF stimulation. In addition, zebrafish HBNF was able to enhance extensive neurite outgrowth in zebrafish embryos during embryogenesis. In summary, a feasible in vivo assay for neurite outgrowth was established in zebrafish. (C) 2004 Elsevier Inc. All rights reserved.