Development of high-oil maize haploid inducer with a novel phenotyping strategy
Development of high-oil maize haploid inducer with a novel phenotyping strategy
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DOI：
10.1016/j.cj.2021.07.009
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发表时间：
2022-04-01
期刊：
影响因子：
6.6
通讯作者：
Chen, Shaojiang
中科院分区：
文献类型：
--
作者：
Liu, Chenxu;Li, Jinlong;Chen, Shaojiang
玉米双单倍体育种技术是现代玉米育种的重要手段。单倍体诱导剂决定了单倍体诱导和鉴定的效率。经过几十年的努力，单倍体诱导率（HIR）从祖先单倍体诱导剂stock6的2%左右提高到现代单倍体诱导剂的10%左右。提高籽粒含油量可进一步提高单倍体鉴定效率。利用分子标记辅助选择技术，以单穗单倍体数为表型标准，在联合收割机上选育出了一个高油单倍体诱导系CHOI 4，其平均HIR为15.8%，平均KOC为11%。CHOI4的高KOC可以在鉴定不同背景的单倍体时实现大于90%的平均准确度，与先前的高油单倍体诱导系CHOI3相比具有降低的假发现率和假阴性率。通过对表型选择策略的比较表明，在单倍体诱导系的选育过程中，每穗的单倍体数可以作为一个表型选择的标准。CHOI4可以进一步提高大规模DH育种计划的效率，降低成本。(C)2021中国农业科学院作物科学研究所中国作物学会Elsevier B.V.代表KeAi Communications Co.制作和主持，公司
Doubled haploid (DH) technology is important in modern maize breeding. Haploid inducers determine the efficiency of both haploid induction and identification. It has taken decades to improve the efficiency, haploid induction rate (HIR), from the similar to 2% of the ancestor haploid inducer, stock6, to the similar to 10% of modern haploid inducers. Improvement of kernel oil content (KOC) would further enhance haploid identification efficiency. Using molecular marker-assisted selection, in combine with the number of haploids per ear as phenotypic criterion, we developed a new high-oil haploid inducer line, CHOI4, with a mean HIR of 15.8% and mean KOC of 11%. High KOC of CHOI4 can achieve a mean accuracy greater than 90% in identification of haploids of different backgrounds, with reduced false discovery rates and false negative rates in comparison with the previous high-oil haploid inducer line, CHOI3. Comparison of phenotypic selection strategies suggested that the number of haploids per ear can be used as a phenotyping criterion during haploid inducer line development. CHOI4 could further increase the efficiency of large-scale DH breeding programs with lower cost. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.