Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor.
Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor.
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两种针对人胰岛素样生长因子-I 受体 α 亚基的新型单克隆抗体。
DOI：
10.1006/bbrc.1993.2220
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发表时间：
1993
影响因子：
3.1
通讯作者：
Fujita-Yamaguchi,Y
中科院分区：
文献类型：
--
作者：
Li,SL;Kato,J;Paz,IB;Kasuya,J;Fujita-Yamaguchi,Y
最近，我们报道了三种针对纯化的人胎盘胰岛素样生长因子(IGF)- 1受体的单克隆抗体（mab）。与研究充分的单克隆抗体αIR-3相反，这些抗体刺激IGF-I和IGF-II与受体的结合和DNA合成[Xiong等，Proc.]国家的。学会科学。U.S.A.1992(89), 5356]。在这里，我们描述了另外两种针对igf - 1受体的单克隆抗体，1H7和2C8，它们具有不同于αIR-3或我们之前报道的单克隆抗体的特征。通过免疫印迹法确定，1H7和2C8均与IGF-I受体的α亚基结合。MAb 1H7抑制IGF-I和IGF-II与IGF-I受体的结合，而2C8对两种配体与受体的结合均无影响。当使用表达人IGF-I受体的NIH 3T3细胞检测它们对DNA合成的影响时，1H7抑制基础和IGF-I或IGF-II刺激的DNA合成，而2C8刺激基础DNA合成，但在IGF-I或IGF-II存在时没有协同作用。
Recently, we have reported three monoclonal antibodies (mAbs) against purified human placental insulin-like growth factor (IGF)-I receptors. These antibodies, in contrast to the well-studied mAb αIR-3, stimulate binding of IGF-I and IGF-II to the receptor and DNA synthesis as well [Xiong,et al.,Proc. Natl. Acad. Sci. U.S.A.1992(89), 5356]. Here we describe two additional mAbs, 1H7 and 2C8, against the IGF-I receptor that have characteristics different from either αIR-3 or our previously reported mAbs. Both 1H7 and 2C8 bind to the α subunit of the IGF-I receptor as determined by immunoblotting. MAb 1H7 inhibited the binding of IGF-I and IGF-II to the IGF-I receptor while 2C8 had no effect on the binding of either ligand to the receptor. When their effects on DNA synthesis were examined using NIH 3T3 cells expressing human IGF-I receptors, 1H7 inhibited basal and IGF-I- or IGF-II-stimulated DNA synthesis whereas 2C8 stimulated basal DNA synthesis but provided no synergism in the presence of IGF-I or IGF-II.