Ineffective hemopoiesis in the myelodysplastic syndromes (MDS) as studied by daily in situ observation of colony-cluster formation.
Ineffective hemopoiesis in the myelodysplastic syndromes (MDS) as studied by daily in situ observation of colony-cluster formation.
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通过每日原位观察集落簇形成来研究骨髓增生异常综合征 (MDS) 中的无效造血。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
M. Okuma
中科院分区：
文献类型：
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作者：
M. Ohmori;S. Ohmori;Y. Ueda;Y. Yoshida;M. Okuma
每日原位观察单个增殖细胞，以检查体外无效造血。用甲基纤维素对24例骨髓增生异常综合征（MDS）患者和12例正常人的骨髓单个核细胞（BMMNC）进行粒细胞-巨噬细胞祖细胞（CFU-gm）培养。在培养第3天绘制单个增殖细胞的图谱，并通过每天对每个细胞聚集体中包含的细胞进行原位细胞计数来跟踪其命运，直至第8天。通过回顾性分析细胞的日常生长，发现MDS中非集落形成细胞的比例显著更大，最初增殖，但此后未能增殖，并在培养中退化。显示这些异常生长特征的细胞显然导致无效的粒细胞生成。本方法可用于阐明无效粒细胞生成。
Daily in situ observation of individual proliferating cells was performed to examine ineffective hemopoiesis in vitro. Bone marrow mononuclear cells (BMMNC) from 24 myelodysplastic syndrome (MDS) patients and 12 controls were cultured for granulocyte-macrophage progenitor (CFU-gm) assays using methylcellulose. Individual proliferating cells were mapped at 3 days of culturing and their fates were followed by daily in situ cell counting contained within each cell aggregate until day 8. By retrospective analysis of the daily growth of the cells, a significantly greater proportion of noncolony-forming cells in MDS were found to proliferate initially, but failed to do so thereafter and degenerated in the culture. Cells showing these abnormal growth characteristics apparently contributed to ineffective granulopoiesis. The present method may be useful for clarifying ineffective granulopoiesis.