Differentiation of Xanthomonas campestris pv. phaseoli from Xanthomonas campestris pv. phaseoli var. fuscans by PFGE and RFLP
Differentiation of Xanthomonas campestris pv. phaseoli from Xanthomonas campestris pv. phaseoli var. fuscans by PFGE and RFLP
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DOI：
10.1023/a:1008785809368
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发表时间：
1999-12-01
影响因子：
1.8
通讯作者：
Goodwin, PH
中科院分区：
文献类型：
--
作者：
Chan, JWYF;Goodwin, PH
野油菜黄单胞菌Phaseoli和野油菜X.campestris PV.菜豆变种。豆类细菌枯萎病的致病因子是多聚糖，它们的表型似乎是相同的，只是后者能在培养中产生一种类似黑色素的色素。10株野油菜X.campestris PV.Phaseoli和12株野油菜X.campestris PV.菜豆变种。采用脉冲场凝胶电泳法(PFGE)和限制性片段长度多态性(RFLP)技术对融合子进行检测。野油菜黄杆菌的平均基因组大小。Phaseoli和野油菜X.campestris PV.菜豆变种。融合蛋白大小分别为3850.6+/-48.9kb和3584.3+/-68.1kb。根据XbaI产生的宏观限制性内切酶图谱，确定这些分离物的遗传亲缘关系。聚类分析表明，非发酵型菌株和发酵型菌株具有明显的差异。基于Xanthomonas高度保守的hrp基因和果胶裂解酶基因的RFLP结果也表明，这两种细菌在遗传上是不同的。本研究的结果表明，根据最近提出的对黄单胞菌属的重新分类，该致病变种可以分为两个亚组。
Xanthomonas campestris pv. phaseoli and X. campestris pv. phaseoli var. fuscans, the causal agents of the common and fuscous bacterial blight of beans, appear to be phenotypically identical except that the latter can produce a melanin-like pigment in culture. Ten isolates of X. campestris pv. phaseoli and 12 isolates of X. campestris pv. phaseoli var. fuscans were examined using pulsed-field gel electrophoresis (PFGE) and restriction fragment length polymorphism (RFLP). The average genome sizes for X. campestris pv. phaseoli and X. campestris pv. phaseoli var. fuscans were 3850.6 +/- 48.9 and 3584.3 +/- 68.1 kb respectively. The genetic relatedness of the isolates was determined from macrorestriction patterns generated using XbaI. Cluster analysis indicated that the non-fuscous and fuscous strains are distinct. RFLP results, based on the highly conserved hrp genes and a pectate lyase gene from Xanthomonas, also indicated that the two bacteria are genetically different. The results obtained in this study suggest that this pathovar can be segregated into two subgroups under a recently proposed reclassification of the Xanthomonas genus.