Residues of CNP Metabolites in Fish and Shellfish
Residues of CNP Metabolites in Fish and Shellfish
复制标题
鱼类和贝类中 CNP 代谢物的残留
DOI：
10.3358/shokueishi.24.187
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发表时间：
1983
影响因子：
0.3
通讯作者：
K. Sakai
中科院分区：
文献类型：
--
作者：
Shigeru Suzuki;N. Sato;K. Takatsuki;E. Kamo;H. Kikuchi;I. Ushizawa;K. Sakai
CNP（氯硝草醚）在日本广泛用作水稻除草剂，已知通过还原环境中的硝基容易转化为相应的氨基衍生物。据报告，在施用期间，淡水鱼类和贝类中CNP残留浓度异常高。因此，研究了高CNP残留水平样品中CNP还原产物的发生情况，并检测到两种未知化合物，其与GLC上的任何标准农药均不相同。这两个化合物，在制备规模的实验中，显示出高灵敏度的ECD-GC和NP-FID GC和CNP-氨基化合物在碱性水解。根据GC-MS分析和上述化学性质，确定这些化合物的结构为CNP-乙酰胺（CNP-a）和CNP-甲酰胺（CNP-f）;通过将产品与CNP制备的真实样品进行比较，确证了这些结构。建立了残留CNP-a和CNP-f的分析方法，并在新鲜样品中研究了残留浓度。全年定期在宫城县的固定地点养鱼和贝类。在5 ~ 6月间，CNP衍生物在河蚌体内的最大浓度分别为0.58ppm（CNP-a）和0.24ppm（CNP-f），在泥鳅体内的最大浓度分别为0.04 ppm（CNP-a）和0.05ppm（CNP-f），在鲫鱼体内的最大浓度分别为0.06 ppm（CNP-a）和0.13ppm（CNP-f）。
CNP (chlornitrofen), widely used as a paddy herbicide in Japan, is known to be converted readily to the corresponding amino derivative by reduction of the nitro group in the environment. An unusually high level of CNP residue concentration was reported in fresh-water fish and shellfish during the application period. Therefore, the occurrence of CNP reduction products in samples with high CNP residue levels was investigated and two unknown compounds were detected which were not identical with any standard pesticides on GLC. These two compounds, obtained in preparative scale experiments, showed high sensitivity in ECD-GC and NP-FID GC and gave CNP-amino compound on alkaline hydrolysis. From the GC-MS analysis and the above mentioned chemical property, the structures of these compounds were elucidated as CNP-acetamide (CNP-a) and CNP-formamide (CNP-f); these structures were confirmed by comparison of the products with authentic samples prepared from CNP.Analytical methods for residual CNP-a and CNP-f were established and the residual concentrations were investigated in fresh-water fish and shellfish at regular intervals through a year at a fixed location in Miyagi prefecture. The maximum concentrations of CNP derivatives were 0.58ppm (CNP-a) and 0.24ppm (CNP-f) in freshwater clam, 0.04 (CNP-a) and 0.05ppm (CNP-f) in loach, and 0.06 (CNP-a) and 0.13ppm (CNP-f) in crucian carp during May and June, and these levels decreased rapidly after the end of the herbicide application period.