Short-term and long-term vitamin C supplementation in humans dose-dependently increases the resistance of plasma to ex vivo lipid peroxidation.
Short-term and long-term vitamin C supplementation in humans dose-dependently increases the resistance of plasma to ex vivo lipid peroxidation.
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DOI：
10.1016/j.abb.2003.12.019
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发表时间：
2004-03
影响因子：
3.9
通讯作者：
M. Polidori;P. Mecocci;M. Levine;B. Frei
中科院分区：
文献类型：
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作者：
M. Polidori;P. Mecocci;M. Levine;B. Frei
为了评估短期和长期补充维生素C对人体血浆抗氧化状态和抗氧化应激的影响，在口服2g维生素C之前和之后2小时从20名个体或从8名参与维生素C消耗-补充研究的受试者中获得血浆，该研究使用每日剂量从30 mg增加到2500 mg的维生素C。抗坏血酸的血浆浓度，而不是其他生理抗氧化剂，在短期补充后显着增加，并在长期研究中逐渐增加，维生素C剂量高达1000毫克/天。孵育后的血浆与自由基引发剂，抗坏血酸浓度呈正相关的滞后期之前可检测到的脂质过氧化。我们的结论是，维生素C补充剂在人类剂量依赖性地增加血浆抗坏血酸浓度，因此，抗血浆脂质过氧化离体。人体血浆和体内维生素C饱和似乎是最大限度地提高抗氧化保护和降低氧化损伤风险的理想选择。
To assess the effects of short-term and long-term vitamin C supplementation in humans on plasma antioxidant status and resistance to oxidative stress, plasma was obtained from 20 individuals before and 2h after oral administration of 2g of vitamin C, or from eight subjects enrolled in a vitamin C depletion–repletion study using increasing daily doses of vitamin C from 30 to 2500mg. Plasma concentrations of ascorbate, but not other physiological antioxidants, increased significantly after short-term supplementation, and increased progressively in the long-term study with increasing vitamin C doses of up to 1000mg/day. Upon incubation of plasma with a free radical initiator, ascorbate concentrations were positively correlated with the lag phase preceding detectable lipid peroxidation. We conclude that vitamin C supplementation in humans dose-dependently increases plasma ascorbate concentrations and, thus, the resistance of plasma to lipid peroxidation ex vivo. Plasma and body saturation with vitamin C in humans appears desirable to maximize antioxidant protection and lower risk of oxidative damage.