Identification of the products of bacteriophage P22 genes, including a new late gene.
Identification of the products of bacteriophage P22 genes, including a new late gene.
复制标题
噬菌体P22基因产物的鉴定，包括一个新的晚期基因。
DOI：
10.1016/0042-6822(80)90291-3
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发表时间：
1980
期刊：
影响因子：
3.7
通讯作者：
Susskind,MM
中科院分区：
文献类型：
--
作者：
Youderian,P;Susskind,MM
本文报道了用SDS-聚丙烯酰胺凝胶电泳鉴定几种噬菌体P22基因的产物。P22早期genesint、erf、17、18、12和23的产物通过它们在用P22野生型噬菌体感染的细胞的放射性标记的裂解物中的存在和在用这些基因中携带琥珀突变的p22感染的细胞的裂解物中的不存在来鉴定。还使用琥珀突变体鉴定了宿主细胞裂解所必需的两个P22晚期基因（基因13和19）的产物。我们还报告了琥珀突变体的分离和鉴定，定义了一个以前未知的P22晚期基因，基因14，并确定了这个新基因的蛋白质产物。P22基因14琥珀突变体与大肠杆菌噬菌体T4基因40无义突变体一样，具有仅在高温下才表现出条件致死表型的特性。这些和以前确定的P22基因产物的表观分子量已被确定。所有已知的必需P22基因的产物，除基因24外，现在已经通过SDS-聚丙烯酰胺凝胶电泳鉴定。
We report the identification of the products of several bacteriophage P22 genes by SDS-polyacrylamide gel electrophoresis. The products of P22 early genesint, erf, 17, 18, 12, and 23 are identified by their presence in radioactively labeled lysates of cells infected with P22 wild-type phage and absence in lysates of cells infected with phages carrying amber mutations in these genes. The products of the two P22 late genes essential for host cell lysis, genes 13 and 19, are also identified using amber mutants. We also report the isolation and characterization of amber mutants defining a previously unknown P22 late gene, gene 14, and identify the protein product of this new gene. P22 gene 14 amber mutants, like coliphage T4 gene 40 nonsense mutants, have the unusual property that they display a conditional-lethal phenotype only at high temperature. The apparent molecular weights of these and previously identified P22 gene products have been determined. The products of all known essential P22 genes, with the exception of gene 24, have now been identified by SDS-polyacrylamide gel electrophoresis.