Light sheet imaging and interactive analysis of the cardiac structure in neonatal mice.
Light sheet imaging and interactive analysis of the cardiac structure in neonatal mice.
复制标题
新生小鼠心脏结构的光片成像和交互式分析。
DOI：
10.1002/jbio.202200278
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发表时间：
2023
影响因子：
2.8
通讯作者：
Ding,Yichen
中科院分区：
文献类型：
--
作者：
Sodimu,Oluwatofunmi;Almasian,Milad;Gan,Peiheng;Hassan,Sohail;Zhang,Xinyuan;Liu,Ning;Ding,Yichen
光片显微镜（LSM）使我们能够加强对哺乳动物模型中心脏发育、损伤和再生的理解。这种新兴技术将激光照明和荧光检测分离开来，以高空间分辨率研究心脏的微观结构和功能，同时最大限度地减少光损伤和最大限度地提高穿透深度。为了揭示体积成像在心脏发育和修复中的潜力，我们试图将我们的内部LSM， Adipo - Clear和虚拟现实（VR）与新生小鼠心脏结合起来。我们演示了使用Adipo‐Clear使小鼠心脏透明，开发我们的内部LSM来捕获完整心脏内的心肌结构，并集成VR以交互式方式探索、测量和评估感兴趣的区域。总的来说，我们已经建立了一种创新的整体图像采集和解释策略，为整个哺乳动物心脏的心肌微结构评估提供了一个切入点，以了解心脏形态发生。
Light‐sheet microscopy (LSM) enables us to strengthen the understanding of cardiac development, injury, and regeneration in mammalian models. This emerging technique decouples laser illumination and fluorescence detection to investigate cardiac micro‐structure and function with a high spatial resolution while minimizing photodamage and maximizing penetration depth. To unravel the potential of volumetric imaging in cardiac development and repair, we sought to integrate our in‐house LSM, Adipo‐Clear, and virtual reality (VR) with neonatal mouse hearts. We demonstrate the use of Adipo‐Clear to render mouse hearts transparent, the development of our in‐house LSM to capture the myocardial architecture within the intact heart, and the integration of VR to explore, measure, and assess regions of interests in an interactive manner. Collectively, we have established an innovative and holistic strategy for image acquisition and interpretation, providing an entry point to assess myocardial micro‐architecture throughout the entire mammalian heart for the understanding of cardiac morphogenesis.