High frequency of the expression of the MAGE gene family in human esophageal carcinoma.
High frequency of the expression of the MAGE gene family in human esophageal carcinoma.
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MAGE基因家族在人食管癌中的高表达频率。
DOI：
10.3892/ijo.10.6.1113
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发表时间：
1997
影响因子：
5.2
通讯作者：
T. Akiyoshi
中科院分区：
文献类型：
--
作者：
F. Tanaka;M. Mori;J. Li;T. Fujie;K. Mimori;M. Haraguchi;Y. Tanaka;K. Mafune;T. Akiyoshi
人类基因法师编码肿瘤特异性肽抗原，至少由12个家族组成。由法师基因编码的某些抗原可能用于癌症特异性免疫治疗。然而，到目前为止，这些基因家族在人食管癌中的表达信息很少。我们用逆转录聚合酶链反应（RT-PCR）方法检测了24株人食管癌细胞系和50对食管癌组织及相应正常组织标本中法师-1、-2、-3、-4、-6、-8、-9、-10、-11和-12基因的表达。食管癌细胞系中法师-6的表达率为13%，法师-4的表达率为8%~ 79%，临床肿瘤组织中法师-6的表达率为6%~ 62%。在细胞系和临床样品中，最常见和最少表达的基因分别是法师-4和法师-6基因。50种临床肿瘤中有47种表达至少一种法师基因。无论是否存在法师基因表达，在肿瘤病例之间均未观察到显著的临床病理学差异。因此，本研究的结果表明，法师基因家族经常在临床样品中表达，以及在人食管癌的细胞系。因此，鉴定法师基因家族不仅对食管肿瘤的特异性免疫治疗，而且对分子诊断用途也是有用的。
The human gene MAGE encodes tumor specific peptide antigens and consists of at least 12 families. Some antigens coded by the MAGE genes may be potentially useful for cancer specific immunotherapy. There is, however, so far little information on the expression of these gene families in human esophageal carcinomas. We investigated the expression of MAGE-1, -2, -3, -4, -6, -8, -9, -10, -11, and -12 genes in 24 human esophageal carcinoma cell lines, and in 50 pairs of tumor and corresponding normal tissue specimens from the human esophagus by means of reverse transcription polymerase chain reaction. The expression rate varied from 13% of MAGE-6 and 8 to 79% of MAGE-4 in the esophageal carcinoma cell lines, and from 6% of MAGE-6 to 62% of MAGE-4 in clinical tumor samples. The most frequently and the least expressed gene were the MAGE-4 and MAGE-6 genes, respectively, in both the cell lines and the clinical samples. Forty-seven of the 50 clinical tumors expressed at least one MAGE gene. No significant clinicopathologic difference between the tumor cases was observed, regardless of the presence or absence of MAGE gene expression. The findings of this study thus demonstrated that the MAGE gene family is frequently expressed in clinical samples as well as in the cell lines of human esophageal carcinomas. Therefore, to identify the MAGE gene family may be useful, not only for esophageal tumor specific immunotherapy but for molecular diagnostic usage as well.