Frizzled-9 promoter drives expression of transgenes in the medial wall of the cortex and its chief derivative the hippocampus
Frizzled-9 promoter drives expression of transgenes in the medial wall of the cortex and its chief derivative the hippocampus
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DOI：
10.1002/gene.20058
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发表时间：
2004-09-01
期刊：
影响因子：
1.5
通讯作者：
Pleasure, SJ
中科院分区：
文献类型：
--
作者：
Zhao, CJ;Pleasure, SJ
在胚胎时期，海马体从形成的大脑皮层的内侧壁发育而来。来自WRIT通路的形态发生信号调节海马发育过程中的几个事件(Galceran等人：Development 127：469-482,2000；Lee等人：Development 127：457-467,2000；周等人：J Neurosci 24：121-126,2004)，我们先前已经证明，来自FrizzledFZD家族的WRIT受体在发育过程中在谨慎的皮质区域表达(Kim等人：Mech Dev 103：167-172,2001)。我们使用Fzd9基因的假定控制元件产生了转基因小鼠，Fzd9基因通常在发育中的和成年的海马区选择性表达，驱动一个标记基因的表达。这些小鼠在大脑中表达LacZ，其发育分布与内源性Fzd蛋白相同。出生后，在海马主细胞和传出轴突的树突区仍有较强的表达。这些小鼠为分析海马体的发育和重组提供了一个遗传和解剖学工具。(C)2004年Wiley-Liss公司
The hippocampus develops from the medial wall of the forming cerebral cortex during embryonic life. Morphogenic signals from the Writ pathway regulate several events during hippocampal development (Galceran et al.: Development 127:469-482, 2000; Lee et al.: Development 127:457-467, 2000; Zhou et al.: J Neurosci 24:121-126, 2004) and we have previously shown that Writ receptors from the Frizzled (Fzd) family are expressed in discreet cortical domains during development (Kim et al.: Mech Dev 103:167-172, 2001). We generated transgenic mice using the putative control elements of the Fzd9 gene, normally selectively expressed in the developing and adult hippocampus, driving expression of a marker gene. These mice express LacZ in the brain in the same developmental distribution as endogenous Fzd protein. Postnatally, expression remains strong in the dendritic fields of hippocampal principal cells as well as hippocampal efferent axons. These mice provide a genetic and anatomic tool for analyzing development and reorganization in the hippocampus. (C) 2004 Wiley-Liss, Inc.