Expression of transient receptor potential vanilloid-1 (TRPV1) in urothelial cancers of human bladder: relation to clinicopathological and molecular parameters
Expression of transient receptor potential vanilloid-1 (TRPV1) in urothelial cancers of human bladder: relation to clinicopathological and molecular parameters
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DOI：
10.1111/j.1365-2559.2010.03683.x
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发表时间：
2010-11-01
期刊：
影响因子：
6.4
通讯作者：
Santoni, Giorgio
中科院分区：
文献类型：
--
作者：
Kalogris, Cristina;Caprodossi, Sara;Santoni, Giorgio
目的：评估瞬时受体电位香草酸1型通道蛋白（TRPV 1）在正常和肿瘤尿路上皮组织中的表达，并将TRPV 1表达与临床病理参数和疾病特异性survival.Methods和结果：TRPV 1在正常和肿瘤尿路上皮样本中的表达进行了分析，在mRNA和蛋白质水平的定量真实的时间聚合酶链反应（qPCR）和免疫组化，分别。在尿路上皮癌（UC）标本中发现TRPV 1下调，这与肿瘤进展相关。此外，TRPV 1 mRNA水平与临床病理参数相关，以评估TRPV 1下调作为生存的负性预后因素的作用。Kaplan-Meier生存分析显示TRPV 1 mRNA下调患者的生存期明显缩短。多变量考克斯回归分析进一步表明，TRPV 1 mRNA表达保留其意义作为一个独立的危险factor.Conclusions：人类膀胱UC的进展与TRPV 1表达的显着下降，与进行性损失在高级肌肉浸润性UC。TRPV 1 mRNA表达下调可能是膀胱癌患者独立的负性预后因素。
Aims:To evaluate the expression of transient receptor potential vanilloid type-1 channel protein (TRPV1) in normal and neoplastic urothelial tissues and to correlate TRPV1 expression with clinicopathological parameters and disease-specific survival.Methods and results:TRPV1 expression was analysed in normal and neoplastic urothelial samples at both mRNA and protein levels by quantitative real time polymerase chain reaction (qPCR) and immunohistochemistry, respectively. TRPV1 downregulation was found in urothelial cancer (UC) specimens, which correlated with tumour progression. Moreover, TRPV1 mRNA levels were associated with clinicopathological parameters to assess the role of TRPV1 downregulation as a negative prognostic factor for survival. Kaplan-Meier survival analysis demonstrated a significantly shorter survival in patients showing TRPV1 mRNA downregulation. Multivariate Cox regression analysis indicated further that TRPV1 mRNA expression retained its significance as an independent risk factor.Conclusions:The progression of UC of human bladder is associated with a marked decrease in TRPV1 expression, with a progressive loss in high-grade muscle invasive UC. Downregulation of TRPV1 mRNA expression may represent an independent negative prognostic factor for bladder cancer patients.