Milling plant and soil material in plastic tubes over-estimates carbon and under-estimates nitrogen concentrations
Milling plant and soil material in plastic tubes over-estimates carbon and under-estimates nitrogen concentrations
复制标题
在塑料管中碾磨植物和土壤材料会高估碳浓度并低估氮浓度
DOI：
10.1007/s11104-013-1599-y
复制
发表时间：
2013
期刊：
影响因子：
4.9
通讯作者：
R. Wal
中科院分区：
文献类型：
--
作者：
Stuart W. Smith;A. Robertson;A. Meharg;R. Pakeman;David Johnson;S. Woodin;R. Wal
背景和目的在塑料管中研磨植物和土壤材料，如微量离心管，高估了碳（C）和低估了氮（N）浓度，由于引入聚丙烯到研磨样品中，如使用傅立叶变换红外光谱法所确定的。证明较长的研磨时间、较大的研磨强度、较小的样品尺寸和包含研磨样品材料都增加了来自塑料管的聚丙烯污染，导致C浓度高估高达8%（0.08 g g−1）。结论C和N以及其他分析物的错误估计，必须假设在塑料管中铣削后，应采用铣削方法以最大限度地减少此类误差。
Background and aimsMilling of plant and soil material in plastic tubes, such as microcentrifuge tubes, over-estimates carbon (C) and under-estimates nitrogen (N) concentrations due to the introduction of polypropylene into milled samples, as identified using Fourier-transform infra-red spectroscopy.Methods and resultsThis study compares C and N concentrations of roots and soil milled in microcentrifuge tubes versus stainless steel containers, demonstrating that a longer milling time, greater milling intensity, smaller sample size and inclusion of abrasive sample material all increase polypropylene contamination from plastic tubes leading to overestimation of C concentrations by up to 8 % (0.08 g g−1).ConclusionsErroneous estimations of C and N, and other analytes, must be assumed after milling in plastic tubes and milling methods should be adapted to minimise such error.