Biomineralized matrix-assisted osteogenic differentiation of human embryonic stem cells.
Biomineralized matrix-assisted osteogenic differentiation of human embryonic stem cells.
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DOI：
10.1039/c4tb00714j
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发表时间：
2014-09-01
期刊：
影响因子：
--
通讯作者：
Varghese S
中科院分区：
文献类型：
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作者：
Kang H;Wen C;Hwang Y;Shih YR;Kar M;Seo SW;Varghese S
基质的物理和化学性质在决定各种细胞行为中起重要作用，包括谱系特异性。我们证明，人胚胎干细胞（hESC）的分化承诺，在体外和体内，可以单独通过合成生物材料实现。使用矿化合成基质培养hESC，所述矿化合成基质模拟富含磷酸钙（CaP）的骨环境，在没有任何成骨诱导补充物的情况下分化成成骨细胞。当植入体内时，这些载有hESC的矿化基质有助于异位骨组织形成。相比之下，相应的非矿化基质内的细胞经历成骨或成脂的命运，这取决于微环境中存在的局部线索。据我们所知，这是第一次证明，其中合成基质显示出诱导终末细胞命运规格的hESC完全由生物材料为基础的线索在体外和体内。利用组织特异性细胞-基质相互作用来控制干细胞命运的技术可能是再生医学的有力工具。这种方法可以作为一种工具，以促进我们的基本理解和评估干细胞的翻译潜力。
The physical and chemical properties of a matrix play an important role in determining various cellular behaviors, including lineage specificity. We demonstrate that the differentiation commitment of human embryonic stem cells (hESCs), both in vitro and in vivo, can be solely achieved through synthetic biomaterials. hESCs were cultured using mineralized synthetic matrices mimicking a calcium phosphate (CaP)-rich bone environment differentiated into osteoblasts in the absence of any osteogenic inducing supplements. When implanted in vivo, these hESC-laden mineralized matrices contributed to ectopic bone tissue formation. In contrast, cells within the corresponding non-mineralized matrices underwent either osteogenic or adipogenic fate depending upon the local cues present in the microenvironment. To our knowledge, this is the first demonstration where synthetic matrices are shown to induce terminal cell fate specification of hESCs exclusively by biomaterial-based cues both in vitro and in vivo. Technologies that utilize tissue specific cell-matrix interactions to control stem cell fate could be a powerful tool in regenerative medicine. Such approaches can be used as a tool to advance our basic understanding and assess the translational potential of stem cells.