ELECTRON MICROSCOPE OBSERVATIONS ON THE STRUCTURE OF THE MYELINATED NERVE FIBER SHEATH
ELECTRON MICROSCOPE OBSERVATIONS ON THE STRUCTURE OF THE MYELINATED NERVE FIBER SHEATH
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DOI：
10.1016/0014-4827(50)90056-5
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发表时间：
1950-01-01
影响因子：
3.7
通讯作者：
FERNANDEZMORAN, H
中科院分区：
文献类型：
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作者：
FERNANDEZMORAN, H
可以制备足够薄的未包埋、固定和新鲜神经纤维的冷冻切片，以进行电子显微镜检查。其中描述了对纽曼、Borysko和Swerdlow的热膨胀技术的改进。用锇酸或福尔马林固定的大鼠坐骨神经纤维的纵向和横向冷冻切片的检查揭示了以下结构。50埃厚，具有特征性的粒状表面结构，50-100埃高。通过神经纤维的横切面显示2个不同的纤维层紧密粘附在鞘的中央板层部分：（a）外部颗粒膜，覆盖有典型的胶原纤维，对应于神经膜和邻近的结缔组织。一种特殊类型的极细的光滑原纤维，100埃厚，没有可检测的周期性，以紧凑的股线结合在一起，经常被观察到附着在该外层的胶原原纤维上。(b)一层紧密编织的细纤维（100埃厚），连接髓鞘和轴突的纤维结构。轴突含有细的纤维网络，其宽度约为100 A，并在横向或斜横向方向上伸展。
Frozen sections of unembedded, fixed and fresh nerve fibers which are thin enough for electron microscopy can be prepd. with a described modification of the thermal-expansion technique of Newman, Borysko and Swerdlow. Examination of longitudinal and transverse frozen sections of rat sciatic nerve fibers fixed in osmic acid or formalin discloses the following structures The bulk of the myelin sheath breaks down into piles of thin, trasparent and flexible lamellae, approx. 50 A thick, with a characteristic granular surface structure, 50-100 A high. Transverse sections through nerve fibers reveal 2 distinct fibrous layers adhering closely to the central, lamellar portion of the sheath: (a) an external granular membrane covered with typical collagen fibrils and corresponding to the Neurilemma and the adjacent connective tissue. A special type of extremely thin smooth fibrils, 100 A thick and with no detectable periodicity, bound together in compact strands, is regularly seen attached to the collagen fibrils of this outer layer. (b) An internal layer of densely woven thin fibrils (100 A thick) which connects the myelin sheath with the fibrous structures of the axon. The axon contains a fine network of fibrils which are approximately 100 A in width and are stretched out in a transverse or oblique transverse direction.