STABLE ISOTOPES OF CARBON, NITROGEN AND HYDROGEN IN THE CONTEMPORARY NORTH-AMERICAN HUMAN FOOD WEB
STABLE ISOTOPES OF CARBON, NITROGEN AND HYDROGEN IN THE CONTEMPORARY NORTH-AMERICAN HUMAN FOOD WEB
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DOI：
10.1080/03670244.1986.9990922
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发表时间：
1986-01-01
影响因子：
1.8
通讯作者：
KAPLAN, IR
中科院分区：
文献类型：
--
作者：
SCHOELLER, DA;MINAGAWA, M;KAPLAN, IR
对生活在美国大都市芝加哥的10名健康受试者使用稳定的碳、氮和氢同位素作为膳食标记进行了调查。对40种食物进行了同位素分析，并与人类血浆和头发进行了比较。根据同位素分析和美国平均食物摄入量数据，膳食蛋白质的同位素组成估计为δ 13 CPDB =-18.0 ‰，δ 13 NAIR = 5.2‰，δ 2 HSMOW =-69 ‰。膳食脂肪估计为δ 13 C =-22.5 ‰，膳食碳水化合物估计为δ 13 C =-19.6 ‰。作为比较，血浆蛋白的同位素组成±SD为δ 13 C =-17.9±0.7‰，δ 13 N = 9.4±0.5‰，2 H =-57±5‰。血浆脂质为δ 13 C =-21.6 ±1.0‰，血浆碳水化合物为δ 13 C =-19.7 ±1.9‰。头发中氮和氢的同位素组成相似，分别为δ 15 N = 9.6±0.5‰和δ 2 H =-62 ±4‰，而碳在δ 13 C中略有富集，13 C =-16.6 ±0.6。相对于食物，组织中15 N的富集可能反映了活体同位素分馏。当比较在同一类常量营养素内时，观察到的碳的饮食/组织差异很小。碳同位素丰度是C4植物碳来源的准确预测因子。
The use of stable carbon, nitrogen and hydrogen isotopes as dietary markers was investigated in ten healthy subjects living in metropolitan Chicago, USA. Forty foods were isotopically analyzed and compared with human plasma and hair. From isotopic analysis and average U.S. food intake data, the isotopic composition of dietary protein was estimated to be δ13CPDB= ‐18.0‰, δ13NAIR= 5.2‰, and δ2HSMOW= ‐69‰. Dietary lipid was estimated to be δ13C = ‐22.5‰ and dietary carbohydrate to be δ13C = ‐19.6‰. For comparison, the isotopic composition ±SD of plasma protein was δ13C = ‐17.9±0.7‰, δ13N = 9.4±0.5‰, and2H = ‐57±5‰. Plasma lipid was δ13C = ‐21.6±1.0‰ and plasma carbohydrate was δ13C = ‐19.7±1.9‰. The isotopic composition of nitrogen and hydrogen in hair was similar, δ15N = 9.6±0.5‰ and δ2H=‐62±4‰ respectively, while carbon was slightly enriched in δ13C,13C = ‐16.6±0.6. The enrichment of15N in tissue relative to diet probably reflectsin vivoisotope fractionation. The diet/tissue differences observed for carbon were small when comparisons were within the same class of macronutrients. The carbon isotope abundance was an accurate predictor of carbon derived from C4plants.