NUCLEOTIDE-SEQUENCE AND CHARACTERIZATION OF THE MAREKS-DISEASE VIRUS HOMOLOG OF GLYCOPROTEIN-B OF HERPES-SIMPLEX VIRUS
NUCLEOTIDE-SEQUENCE AND CHARACTERIZATION OF THE MAREKS-DISEASE VIRUS HOMOLOG OF GLYCOPROTEIN-B OF HERPES-SIMPLEX VIRUS
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DOI：
10.1099/0022-1317-70-7-1789
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发表时间：
1989-07-01
影响因子：
3.8
通讯作者：
MILNE, B
中科院分区：
文献类型：
--
作者：
ROSS, LJN;SANDERSON, M;MILNE, B
编码糖蛋白 B (gB) 的单纯疱疹病毒 (HSV) 基因的马立克氏病病毒 (MDV) 同源物已在 MDV 的“高致癌性”菌株 RB1B 的 BamHI 片段 I3 和 K3 中被鉴定。该基因的完整核苷酸序列已被确定，其预测的氨基酸序列显示出与HSV、水痘带状疱疹病毒(VZV)和其他哺乳动物疱疹病毒的gB基因共有总体结构特征。特别是，所有 10 个半胱氨酸残基在 MDV gB 中都是保守的，并且除 N 和 C 末端外，整个基因与 VZV、HSV 和伪狂犬病病毒具有广泛的同源性。 MDV gB 和α-疱疹病毒 gB 之间的总体氨基酸同一性百分比平均值为 50%，几乎是巨细胞病毒和 Epstein-Barr 病毒之间的两倍。 Northern印迹分析显示该基因转录的主要RNA约为。大小为 2.7 kb。针对合成肽（残基 250 至 271 和 304 至 330）产生的抗体允许使用免疫印迹鉴定 MDV 感染细胞提取物中 Mr 110K、64K 和 48K 的血清学相关糖蛋白家族。此外，抗血清能够在免疫印迹中区分火鸡的 MDV 和疱疹病毒抗原。免疫荧光测试表明，MDV gB 与细胞质中的颗粒相关，并且存在于 MDV 感染细胞的表面。
The Marek''s disease virus (MDV) homologue of the herpes simplex virus (HSV) gene encoding glycoprotein B (gB) has been identified within BamHI fragments I3 and K3 of the ''highly oncogenic'' strain RB1B of MDV. The entire nucleotide sequence of the gene has been determined and its predicted amino acid sequence shown to share gross overall structural features with the gB genes of HSV, varicella-zoster virus (VZV) and other mammalian herpesviruses. In particular, all 10 cysteine residues were conserved in MDV gB and there was extensive homology throughout the gene with VZV, HSV and pseudorabies virus except for the N and C termini. The overall percentage amino acid identity between MDV gB and gB of the alphaherpesviruses had a mean of 50% which was almost twice that between cytomegalovirus and Epstein-Barr virus. Northern blot analysis showed that the main RNA transcribed from this gene is approx. 2.7 kb in size. Antibodies raised against synthetic peptides (residues 250 to 271 and 304 to 330) allowed the identification of a family of serologically related glycoproteins of Mr 110K, 64K and 48K in extracts of MDV-infected cells using immunoblots. Furthermore, the antisera were able to differentiate between the antigens of MDV and herpesvirus of turkeys in immunoblots. Immunofluorescence tests indicated that MDV gB is associated with granules in the cytoplasm and is present at the surface of MDV-infected cells.