In vitro bone formation using muscle-derived cells: a new paradigm for bone tissue engineering using polymer-bone morphogenetic protein matrices
In vitro bone formation using muscle-derived cells: a new paradigm for bone tissue engineering using polymer-bone morphogenetic protein matrices
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DOI：
10.1016/s0006-291x(03)00858-1
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发表时间：
2003-06-13
影响因子：
3.1
通讯作者：
Laurencin, CT
中科院分区：
文献类型：
--
作者：
Lu, HH;Kofron, MD;Laurencin, CT
在美国每年进行超过800，000例骨移植手术，产生了对自体骨（骨修复中的移植标准）的可行替代品的需求。本研究的目的是检查BMP-聚合物基质在诱导成骨细胞表型表达和肌源性细胞体外骨形成中的功效。具体而言，我们评估了骨形态发生蛋白-7（BMP-7）的能力，从聚（丙交酯-共-乙交酯）（PLAGA）矩阵，诱导分化的细胞来源于兔骨骼肌成骨细胞样细胞，随后形成矿化组织。结果证实，肌源性细胞贴附和增殖的PLAGA基板上。从PLAGA释放的BMP-7诱导肌源性细胞增加骨标记物表达并形成矿化培养物。这些结果证明了BMP-聚合物基质诱导肌源性细胞表达成骨细胞表型的功效，并为骨组织工程提供了新的范例。(C)2003 Elsevier Science（美国）。All rights reserved.
Over 800,000 bone grafting procedures are performed in the United States annually, creating a demand for viable alternatives to autogenous bone, the grafting standard in osseous repair. The objective of this study was to examine the efficacy of a BMP-polymer matrix in inducing the expression of the osteoblastic phenotype and in vitro bone formation by muscle-derived cells. Specifically, we evaluated the ability of bone morphogenetic protein-7 (BMP-7), delivered from a poly (lactide-co-glycolide) (PLAGA) matrix, to induce the differentiation of cells derived from rabbit skeletal muscle into osteoblast-like cells and subsequently form mineralized tissue. Results confirmed that muscle-derived cells attached and proliferated on the PLAGA substrates. BMP-7 released from PLAGA induced the muscle-derived cells to increase bone marker expression and form mineralized cultures. These results demonstrate the efficacy of a BMP-polymer matrix in inducing the expression of the osteoblastic phenotype by muscle-derived cells and present a new paradigm for bone tissue engineering. (C) 2003 Elsevier Science (USA). All rights reserved.