Formation and remodeling of the coronary vascular bed in the embryonic avian heart
Formation and remodeling of the coronary vascular bed in the embryonic avian heart
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DOI：
10.1002/dvdy.20022
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发表时间：
2004-05-01
影响因子：
2.5
通讯作者：
Bristow, J
中科院分区：
文献类型：
--
作者：
Kattan, J;Dettman, RW;Bristow, J
为了研究禽类心脏冠脉血管的形成，我们用内皮特异性抗体QH-1对发育阶段鹌鹑心脏进行染色。在汉堡包和汉密尔顿期（HH） 17胚胎中，OH-1仅在接触心脏后才与心外膜前器官中的单个细胞发生反应。在HH18-26心脏中，个别OH-1细胞积聚在心房和心室表面。第一批内皮血管出现在HH23心脏的房室沟背侧。CD45（+）造血前体在心脏表面积累，表明心脏发育过程中造血与血管发生的时间关系密切。然而，CD45的表达先于这些细胞与脉管系统的关联，表明造血功能先于冠状动脉脉管系统血岛的形成。内皮小管首先出现在心脏的背侧，然后是腹侧，合并成大的窦状体。这些窦状体被HH35重塑成致密的肌肉化血管。平滑肌细胞标记物；首先在HH27表达，并且只与血管的发育有关。我们没有观察到心外膜前平滑肌分化的标志物，但仍不确定心外膜前的细胞是否致力于这种细胞命运。我们的数据严格支持冠状血管和血岛形成的血管生成机制。(C) 2004 Wiley-Liss, Inc。
To study the formation of the coronary vessels in the developing avian heart, we stained developmentally staged quail hearts with the endothelial specific antibody QH-1. OH-1 reacted with individual cells in the proepicardial organ in Hamburger and Hamilton stage (HH) 17 embryos only after it had contacted the heart. In HH18-26 hearts, individual OH-1 cells accumulated over the surface of the atria and ventricles. The first endothelial vessels appeared in the dorsal atrioventricular groove in HH23 hearts. CD45(+) hematopoietic precursors accumulated on the heart surface, demonstrating the close temporal relationship of hematopoiesis with vasculogenesis during heart development. However, CD45 expression preceded association of these cells with the vasculature, suggesting hematopoietic commitment precedes formation of blood islands in the coronary vasculature. Endothelial tubules first appeared on the dorsal and then the ventral aspects of the heart, coalescing into large sinusoids. These sinusoids remodeled into compact muscularized vessels by HH35. Smooth muscle cell markers; were first expressed at HH27 and only in association with developing vasculature. We did not observe markers of smooth muscle differentiation in the proepicardium, but it remains uncertain whether cells in the proepicardium are committed to this cell fate. Our data support a strictly vasculogenic mechanism for the formation of the coronary vessels and blood islands. (C) 2004 Wiley-Liss, Inc.