Pathogen-induced production of the antifungal AFP protein from Aspergillus giganteus confers resistance to the blast fungus Magnaporthe grisea in transgenic rice
Pathogen-induced production of the antifungal AFP protein from Aspergillus giganteus confers resistance to the blast fungus Magnaporthe grisea in transgenic rice
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DOI：
10.1094/mpmi-18-0960
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发表时间：
2005-09-01
影响因子：
3.5
通讯作者：
San Segundo, B
中科院分区：
文献类型：
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作者：
Moreno, AB;Peñas, G;San Segundo, B
稻瘟病是由稻瘟菌（Magnaporthe grisea）引起的世界范围内栽培稻最重要的真菌病害。我们已经开发出一种策略，创造抗病性的M。稻瘟病菌，从而在转基因水稻植物中发生病原体诱导的来自黑曲霉的afp（抗真菌蛋白）基因的表达。在这里，我们评估了三个玉米病程相关（PR）基因，ZmPR 4，mpi，PRms，在转基因水稻的启动子的活性。在玉米启动子和β-葡萄糖醛酸酶报告基因（gus A）之间制备嵌合基因融合体。转基因水稻中GUS活性的组织化学测定显示，ZmPR 4启动子在真菌感染、真菌诱导子处理和机械创伤中被强烈诱导。ZmPR 4启动子在种子胚乳中没有活性。mpi启动子也被证明对真菌感染和创伤有反应，但对激发子处理无反应。相反，在转基因水稻叶片中没有观察到PRms启动子的活性。产生在ZmPR 4启动子控制下表达afp基因的转基因植物。转化子对M.在不同层次上。我们的研究结果表明，病原菌诱导的afp基因在水稻植株中的表达可能是一种实用的抗稻瘟病的方法。
Rice blast, caused by Magnaporthe grisea, is the most important fungal disease of cultivated rice worldwide. We have developed a strategy for creating disease resistance to M. grisea whereby pathogen-induced expression of the afp (antifungal protein) gene from Aspergillus giganteus occurs in transgenic rice plants. Here, we evaluated the activity of the promoters from three maize pathogenesis-related (PR) genes, ZmPR4, mpi, and PRms, in transgenic rice. Chimeric gene fusions were prepared between the maize promoters and the beta-glucuronidase reporter gene (gus A). Histochemical assays of GUS activity in transgenic rice revealed that the ZmPR4 promoter is strongly induced in response to fungal infection, treatment with fungal elicitors, and mechanical wounding. The ZmPR4 promoter is not active in the seed endosperm. The mpi promoter also proved responsiveness to fungal infection and wounding but not to treatment with elicitors. In contrast, no activity of the PRms promoter in leaves of transgenic rice was observed. Transgenic plants expressing the afp gene under the control of the ZmPR4 promoter were generated. Transformants showed resistance to M. grisea at various levels. Our results suggest that pathogen-inducible expression of the afp gene in rice plants may be a practical way for protection against the blast fungus.