HUMAN ERYTHROCYTE MEMBRANE GLYCOPROTEIN - RE-EVALUATION OF MOLECULAR WEIGHT AS DETERMINED BY SDS POLYACRYLAMIDE GEL ELECTROPHORESIS
HUMAN ERYTHROCYTE MEMBRANE GLYCOPROTEIN - RE-EVALUATION OF MOLECULAR WEIGHT AS DETERMINED BY SDS POLYACRYLAMIDE GEL ELECTROPHORESIS
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DOI：
10.1016/0006-291x(71)90612-7
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发表时间：
1971-01-01
影响因子：
3.1
通讯作者：
MARCHESI, VT
中科院分区：
文献类型：
--
作者：
SEGREST, JP;JACKSON, RL;MARCHESI, VT
在 SDS 丙烯酰胺凝胶电泳上相对于蛋白质标准计算的人红细胞膜糖蛋白和其他糖蛋白的分子量取决于凝胶的交联百分比。这种异常现象是由于 SDS 与寡糖侧链（相对于多肽主链）的结合减少所致；去除唾液酸无法纠正异常。先前利用 SDS 凝胶电泳提出的人红细胞膜糖蛋白的分子量可能不正确，并且根据修正的 SDS 凝胶数据提出了新值 55,000。
Molecular weights for the human erythrocyte membrane glycoprotein and other glycoproteins calculated relative to protein standards on SDS acrylamide gel electrophoresis depend upon the percent crosslinking of gels. This anomaly is due to a decreased binding of SDS to the oligosaccharide side chains relative to the polypeptide backbone; removal of sialic acid fails to correct the anomaly. Previous molecular weights proposed for the human erythrocyte membrane glycoprotein utilizing SDS gel electrophoresis are probably incorrect and a new value of 55,000 is proposed based on corrected SDS-gel data.