ADULT AND EMBRYONIC MOUSE NEURAL CELL-ADHESION MOLECULES HAVE DIFFERENT BINDING-PROPERTIES
ADULT AND EMBRYONIC MOUSE NEURAL CELL-ADHESION MOLECULES HAVE DIFFERENT BINDING-PROPERTIES
复制标题
DOI：
10.1038/304347a0
复制
发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
GORIDIS, C
中科院分区：
文献类型：
--
作者：
SADOUL, R;HIRN, M;GORIDIS, C
神经细胞表面之间的相互作用在神经个体发生过程中似乎是最重要的，并负责指导迁移的神经母细胞和生长的轴突，以及突触的形成。人们对其潜在的分子机制知之甚少，但大多数假说都暗示了细胞表面分子的存在，这些分子在神经细胞之间形成暂时或永久的键。最近，一种称为神经细胞黏附分子(N-CAM)的膜糖蛋白在鸡1、2和杆状3神经组织中被鉴定，它似乎在神经细胞体4、5或神经元6之间的黏附中扮演着配体的角色。我们已经鉴定出一种小鼠神经表面糖蛋白，命名为BSP-2(参考文献2)。7)，根据电泳迁移7-9、发育变化8、9、氨基酸10和糖11的组成似乎与N-CAM密切相关或相同。BSP-2(参考文献8，9)和N-CAM2，12在脑发育过程中都经历了从胚胎到成体的转换，有人认为这种转换改变了分子的粘附性12或结合特异性。使用神经母细胞瘤细胞系研究胚胎和成人BSP-2/N-CAM分子的功能差异，我们在这里展示了携带成人BSP-2而不是携带胚胎形式的脂质体与神经母细胞瘤细胞黏附，证明了这两种形式确实具有不同的结合特性。
Interactions between neural cell surfaces seem to be of prime importance during neuroontogenesis, and responsible for the guidance of migrating neuroblasts and growing axons and for the formation of synapses. Little is known about the underlying molecular mechanisms, but most hypotheses imply the existence of cell-surface molecules that mediate the formation of transient or permanent bonds between neural cells. Recently, a membrane glycoprotein called neural cell adhesion molecule (N-CAM) has been characterized in chick1,2and rodent3nervous tissue that appears to act as a ligand in adhesion among neural cell bodies4,5or neurites6. We have identified a mouse neural surface glycoprotein, named BSP-2 (ref. 7), which by criteria of electrophoretic migration7–9, developmental changes8,9, amino acid10and sugar11composition seems to be closely related or identical to N-CAM. Both BSP-2 (refs 8,9) and N-CAM2,12undergo conversion from an embryonic to an adult form during brain development and it has been suggested that this transition changes the adhesive properties12or the binding specificity8of the molecule. Using a neuroblastoma line to study functional differences between embryonic and adult BSP-2/N-CAM molecules, we show here that liposomes bearing adult BSP-2 but not those bearing the embryonic form adhere to neuroblastoma cells, demonstrating that the two forms do indeed possess different binding properties.