A Rapid and Sensitive Chemiluminescence Enzyme Linked Immunosorbent Assay for the Determination of Metolcarb Residue in Agricultural Products
A Rapid and Sensitive Chemiluminescence Enzyme Linked Immunosorbent Assay for the Determination of Metolcarb Residue in Agricultural Products
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DOI：
10.1109/icbbe.2009.5162231
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发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
Jingwei Sun;Shuo Wang;Tingting Dong
中科院分区：
文献类型：
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作者：
Jingwei Sun;Shuo Wang;Tingting Dong
建立了一种基于多克隆抗体的酶联免疫吸附-电致发光（ECL）检测食品中速灭威的方法。研究了酶示踪剂的稀释度和包被抗体的浓度。在最佳条件下，测定其IC_（50）值为5 ng·mL ~（-1），ICu值为0.2ng·mL ~（-1）。采用简单、快速、高效的提取方法，完成了橙子汁和蔬菜中速灭威的测定。从加标样品中回收速灭威的范围为79%至99%。通过比较高效液相色谱法（HPLC）的结果进行ECL-ELISA的验证。使用ECL-ELISA和HPLC获得的数据之间的相关性很高。因此，本研究建立的ECL-ELISA方法适用于农产品中速灭威的快速定量检测。
An enzyme linked immunosorbent assay based on polyclonal antibodies with enhanced chemiluminescent (ECL) detection for metolcarb in food is developed. Enzyme tracer dilution and concentration of coating antibody were studied. Under the optimum conditions, the IC50 value and ICu value of assay were determined to be 5 ng mL -1 and 0.2 ng mL -1 respectively. Determination of metolcarb in orange juice and vegetables was accomplished by simple, rapid and efficient extraction methods. Recoveries of metolcarb from spiked samples ranged from 79% to 99%. Validation of the ECL-ELISA was conducted by comparison of results from high performance liquid chromatography (HPLC). The correlation between the data obtained using ECL-ELISA and HPLC was high. Therefore, the ECL-ELISA in this study was suitable for the rapid quantitative determination of metolcarb in agricultural products.