SPECIES-SPECIFIC SERODIAGNOSIS OF EQUINE PIROPLASMA INFECTIONS BY MEANS OF COMPLEMENT-FIXATION TEST (CFT), IMMUNOFLUORESCENCE (IIF), AND ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA)
SPECIES-SPECIFIC SERODIAGNOSIS OF EQUINE PIROPLASMA INFECTIONS BY MEANS OF COMPLEMENT-FIXATION TEST (CFT), IMMUNOFLUORESCENCE (IIF), AND ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA)
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DOI：
10.1016/0304-4017(86)90091-9
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发表时间：
1986-03-01
影响因子：
2.6
通讯作者：
WEILAND, G
中科院分区：
文献类型：
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作者：
WEILAND, G
由于包括美利坚合众国在内的各国实施了进口限制，马匹贸易的增加需要对马螺原体感染进行可靠的免疫诊断。本研究的目的是建立血清学检测方法检测寄生虫携带者的适用性，并最终区分卡氏巴贝斯虫和b型巴贝斯虫。equiinfections。该研究在11匹实验诱导的b型马身上进行。caballiand / orB。equiinfection。感染后2-13天血涂片显示寄生虫，证实感染。采用补体固定试验（CFT）、间接免疫荧光（IIF）和酶联免疫吸附试验（ELISA）对抗体进行验证，并对不同抗原制剂的适用性进行检验。抗体可以通过这三种测试来证明。补体固定抗体在2-3个月PI inB后消失。潜伏感染时，IIF和ELISA检测结果均为阳性。因此，可靠的血清诊断需要使用CFT和IIF，因为寄生虫携带者可能在CFT中表现为血清阴性。b。caballiandB。在早期感染时，CFT和IIF可以在一定程度上达到相等，但ELISA不能。b。caballi感染Berenil®只能通过IIF血清学确诊。
The increasing horse trade requires a reliable immunodiagnosis of equine piroplasma infections due to import restrictions imposed by various countries, including the United States of America. It was the aim of our investigations to establish the suitability of serological tests for the detection of parasite carriers and, eventually, to differentiate betweenBabesia caballiandB. equiinfections. The investigations were carried out on 11 ponies with experimentally-inducedB. caballiand/orB. equiinfection. The infections were confirmed by the demonstration of parasites in blood smears 2–13 days post infection (PI). The complement fixation test (CFT), the indirect immunofluorescence (IIF) and the enzyme-linked immunosorbent assay (ELISA) were employed for the demonstration of antibodies, and different antigen preparations were tested for their suitability. Antibodies could be demonstrated by all three tests. Complement-fixing antibodies disappear after 2–3 months PI inB. caballi-infected horses, while the IIF and ELISA gave positive results during latent infection. A reliable serodiagnosis thus requires the use of the CFT and IIF, since parasite carriers may appear seronegative by the CFT. Serological differentiation betweenB. caballiandB. equiwas possible by CFT and, to a certain extent, by IIF during early infection, but not by ELISA. The successful treatment ofB. caballiinfections with Berenil® could only be confirmed serologically by IIF.