Reproducible gene expression measurement among multiple laboratories obtained in a blinded study using standardized RT (StaRT)-PCR
Reproducible gene expression measurement among multiple laboratories obtained in a blinded study using standardized RT (StaRT)-PCR
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DOI：
10.1054/modi.2001.29789
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发表时间：
2001-12-01
期刊：
影响因子：
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通讯作者：
Willey, JC
中科院分区：
文献类型：
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作者：
Crawford, EL;Peters, GJ;Willey, JC
背景资料：提供标准化数据并且相对便宜且能够高通量的方法是开发适合于基于表达测量进行多机构临床研究的有意义的基因表达数据库的先决条件。标准化RT（StaRT）-PCR具有上述特点。此外，该方法必须具有重现性。StaRT-PCR具有较高的实验室内重复性。本研究的目的是，以确定是否提供类似的实验室间reproducibility.Methods和结果：在一个盲interlaboratory研究，十个基因的表达进行了测量，由StaRT-PCR在互补的DNA样品提供给四个实验室。9个可定量基因的实验室间比较的平均变异系数为0.48。在所有的实验室，表达的基因之一是太低，以measured.Conclusion：由于StaRT-PCR数据是标准化的和数值的方法是可重复的多个实验室，它将允许开发一个有意义的基因表达数据库。
Background: A method that provides standardized data and is relatively inexpensive and capable of high throughput is a prerequisite to the development of a meaningful gene expression database suitable for conducting multi-institutional clinical studies based on expression measurement. Standardized RT (StaRT)-PCR has all these characteristics. In addition, the method must be reproducible. StaRT-PCR has high intralaboratory reproducibility. The purpose of this study is to determine whether StaRT-PCR provides similar interlaboratory reproducibility.Methods and Results: In a blinded interlaboratory study, expression of ten genes was measured by StaRT-PCR in a complementary DNA sample provided to each of four laboratories. The average coefficient of variation for interlaboratory comparison of the nine quantifiable genes was 0.48. In all laboratories, expression of one of the genes was too low to be measured.Conclusion: Because StaRT-PCR data are standardized and numerical and the method is reproducible among multiple laboratories, it will allow development of a meaningful gene expression database.