A novel filamentous phage, fs-2, of Vibrio cholerae O139
A novel filamentous phage, fs-2, of Vibrio cholerae O139
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DOI：
10.1099/00221287-144-7-1901
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发表时间：
1998-07-01
期刊：
影响因子：
2.8
通讯作者：
Honma, Y
中科院分区：
文献类型：
--
作者：
Ikema, M;Honma, Y
一种新的丝状噬菌体fs-2。从霍乱弧菌0139菌株MDO 14中分离得到。fs-2噬菌体是长1200 nm、宽7 nm的长丝状颗粒。当在宿主生物的菌苔上点样时，纯化的噬菌体形成混浊的噬菌斑。空斑形成活性在加热至70 ℃后是稳定的，但被氯仿处理抑制。fs-2具有单链DNA基因组，并在宿主细胞中转化为双链复制形式。几乎所有测试的霍乱弧菌0139和01 EI Tor生物型菌株对噬菌体敏感，但大多数01经典菌株和非01非0139菌株对噬菌体耐药。fs-2基因组包含8651个核苷酸，含有9个开放阅读框，其中5个预测的蛋白质产物与其他丝状真菌的蛋白质产物部分同源。虽然同源性的程度不是特别高，但其他丝状真菌的遗传组织似乎在fs-2中得到了保留。噬菌体没有整合到其宿主的染色体中，但是位于fs-2的大基因间区域中的715个核苷酸片段与霍乱弧菌CTX Phi序列的区域RS 2（重复序列2）的一部分高度同源，推测这是噬菌体在特定位点整合到霍乱弧菌染色体中所需的。
A novel filamentous bacteriophage, fs-2. was isolated from Vibrio cholerae 0139 strain MDO14. The fs-2 phage was a long filamentous particle 1200 nm long and 7 nm wide. The purified phage formed a turbid plaque when spotted on a lawn of the host organisms. The plaque-formation activity was stable following heating to 70 degrees C but was inhibited by treatment with chloroform. fs-2 had a single-stranded DNA genome and was converted to a double-stranded replicative form in the host cell. Almost all V. cholerae 0139 and 01 EI Tor biotype strains tested were sensitive to the phage, but most 01 classical strains and non-01 non-0139 strains were resistant. The fs-2 genome comprised 8651 nucleotides containing nine open reading frames, five of which had predicted protein products partially homologous to the reported protein products of other filamentous phages. Although the extent: of the homology was not particularly high, the genetic organization of other filamentous phages appears to be preserved in fs-2. The phage was not integrated into the chromosome of its host, but a 715 nucleotide fragment located in the large intergenic region of fs-2 was highly homologous to a part of region RS2 (repetitive sequence 2) of the V. cholerae CTX Phi sequence which is speculated to be required for integration of the phage into the V. cholerae chromosome at a specific site.