SONIC DISINTEGRATION OF ISOLATED LIVER MITOCHONDRIA
SONIC DISINTEGRATION OF ISOLATED LIVER MITOCHONDRIA
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DOI：
10.1038/166302a0
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发表时间：
1950-01-01
期刊：
影响因子：
64.8
通讯作者：
SCHNEIDER, WC
中科院分区：
文献类型：
--
作者：
HOGEBOOM, GH;SCHNEIDER, WC
线粒体的已知酶活性导致以9-kc/sec对其进行破坏的实验。声波振动辛酸氧化酶完全失活，琥珀酸氧化酶约50%失活，而DPN-细胞色素还原酶和细胞色素氧化酶的失活率小于20%。在5，000 g下浸泡10 min后，并在148，000 g下再次保持30分钟，58%的总氮仍在上清液中，也有一定的酶活性，但大部分酶活性在2d的沉积物中。上清液组分的沉降常数为4-5、7.2和12.0 Svedberg单位。
Known enzymic activity of mitochondria led to experiment on their disruption by 9-kc/sec. sonic vibrations. Octanoic acid oxidase was completely inactivated; succinoxidase about 50%; while DPN-cytochrome reductase and cytochrome oxidase were inactivated less than 20%. After centrifuging for 10 min. at 5,000 g., and again for 30 min. at 148,000 g., 58% of total N was still in supernatant; also some enzyme activity; but most of enzyme activity was in the 2d sediment. Components of the supernatant gave sedimentation constants of 4-5, 7.2, and 12.0 Svedberg units.