AFLP variation in 25 Avena species
AFLP variation in 25 Avena species
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DOI：
10.1007/s00122-008-0778-3
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发表时间：
2008-08-01
影响因子：
5.4
通讯作者：
Williams, David J.
中科院分区：
文献类型：
--
作者：
Fu, Yong-Bi;Williams, David J.
目前植物移地种质资源的分子鉴定多侧重于培养基因库，较少关注代表野生近缘种的外来基因库。本研究试图通过对不同燕麦品种的种质资源进行鉴定，以期为燕麦育种和研究提供一套参考的外来燕麦种质资源。利用扩增片段长度多态性（AFLP）技术对25种不同地理来源的羊草属163份材料进行了筛选。每次加入，对5对AFLP引物检测到的413个AFLP多态性条带进行评分。多态性频带频率范围为0.006 ~ 0.994，平均为0.468。分子变异分析显示，25个燕麦种间的AFLP变异占59.5%，6个燕麦属鉴定片段间的AFLP变异占45.9%，3个现有倍性水平间的AFLP变异占36.1%，8个已确定的基因组型间的AFLP变异占50.8%。所有种属按其倍性水平聚类。C基因组二倍体最明显，其次是Ac基因组二倍体。Ac基因组似乎是所有A基因组中最古老的，其次是As、Al和Ad基因组。AC基因组四倍体与ACD基因组六倍体的亲缘关系大于AB基因组四倍体。AFLP相似性分析表明，AC基因组四倍体maroccana可能来源于Cp基因组二倍体A. eriantha和As基因组二倍体A. wiestii，并且可能是ACD基因组六倍体的祖先。这些AFLP模式对我们了解燕麦物种和基因组的进化途径，建立外来燕麦种质资源参考集，探索燕麦改良的新外来基因来源具有重要意义。
Current molecular characterization of ex situ plant germplasm has placed more emphasis on cultivated gene pools and less on exotic gene pools representing wild relative species. This study attempted to characterize a selected set of germplasm accessions representing various Avena species with the hope to establish a reference set of exotic oat germplasm for oat breeding and research. The amplified fragment length polymorphism (AFLP) technique was applied to screen 163 accessions of 25 Avena species with diverse geographic origins. For each accession, 413 AFLP polymorphic bands detected by five AFLP primer pairs were scored. The frequencies of polymorphic bands ranged from 0.006 to 0.994 and averaged 0.468. Analysis of molecular variance revealed 59.5% of the total AFLP variation resided among 25 oat species, 45.9% among six assessed sections of the genus, 36.1% among three existing ploidy levels, and 50.8% among eight defined genome types. All the species were clustered together according to their ploidy levels. The C genome diploids appeared to be the most distinct, followed by the Ac genome diploid A. canariensis. The Ac genome seemed to be the oldest in all the A genomes, followed by the As, Al and Ad genomes. The AC genome tetraploids were more related to the ACD genome hexaploids than the AB genome tetraploids. Analysis of AFLP similarity suggested that the AC genome tetraploid A. maroccana was likely derived from the Cp genome diploid A. eriantha and the As genome diploid A. wiestii, and might be the progenitor of the ACD genome hexaploids. These AFLP patterns are significant for our understanding of the evolutionary pathways of Avena species and genomes, for establishing reference sets of exotic oat germplasm, and for exploring new exotic sources of genes for oat improvement.