Lactobacillus plantarum DSM 2648 is a potential probiotic that enhances intestinal barrier function
Lactobacillus plantarum DSM 2648 is a potential probiotic that enhances intestinal barrier function
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DOI：
10.1111/j.1574-6968.2010.02038.x
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发表时间：
2010-08-01
影响因子：
2.1
通讯作者：
Roy, Nicole C.
中科院分区：
文献类型：
--
作者：
Anderson, Rachel C.;Cookson, Adrian L.;Roy, Nicole C.
这项研究的目的是鉴定具有改善肠道屏障功能的潜力的细菌分离株。筛选植物乳杆菌和人口腔分离株，以提高其增强紧密连接完整性的能力，如跨二维尔电阻（TEER）测定法所测量的。比较了八种商业使用的益生菌，以确定哪些对TEER具有最大的积极作用，并且使用最佳性能益生菌菌株HN001乳腺乳杆菌作为评估分离株的基准。选择了一个分离株plantarum dsm 2648，是因为它比鼠李乳杆菌HN001增加了135％，因此选择了进一步的研究。在所有测定中，确定了L. Plantarum dsm 2648耐受胃肠道条件和粘附在肠道细胞上的能力，并且plantarum dsm 2648的性能优于H. rhamnosus hn001。 lactobacillus plantarum dsm 2648能够降低大肠杆菌[肠病大肠杆菌（EPEC）] O127：H6（E2348/69）对TEER和依从性的负面影响，分别降低了多达98.75％和80.18％的负面影响。与仅EPEC孵育相比，先前的共培养。到目前为止，与L. plantarum DSM 2648相关的积极作用相关的确切机制尚不清楚，并且可能影响其用于改善人类健康和健康的用途。
The aim of this research was to identify bacterial isolates having the potential to improve intestinal barrier function. Lactobacillus plantarum strains and human oral isolates were screened for their ability to enhance tight junction integrity as measured by the transepithelial electrical resistance (TEER) assay. Eight commercially used probiotics were compared to determine which had the greatest positive effect on TEER, and the best-performing probiotic strain, Lactobacillus rhamnosus HN001, was used as a benchmark to evaluate the isolates. One isolate, L. plantarum DSM 2648, was selected for further study because it increased TEER 135% more than L. rhamnosus HN001. The ability of L. plantarum DSM 2648 to tolerate gastrointestinal conditions and adhere to intestinal cells was determined, and L. plantarum DSM 2648 performed better than L. rhamnosus HN001 in all the assays. Lactobacillus plantarum DSM 2648 was able to reduce the negative effect of Escherichia coli [enteropathogenic E. coli (EPEC)] O127:H6 (E2348/69) on TEER and adherence by as much as 98.75% and 80.18%, respectively, during simultaneous or prior coculture compared with EPEC incubation alone. As yet, the precise mechanism associated with the positive effects exerted by L. plantarum DSM 2648 are unknown, and may influence its use to improve human health and wellness.