Histomorphology of gastrointestinal tract in bullfrog Rana (Lithobates) catesbeiana and evaluation of the changes induced by a soybean meal-based diet
Histomorphology of gastrointestinal tract in bullfrog Rana (Lithobates) catesbeiana and evaluation of the changes induced by a soybean meal-based diet
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牛蛙胃肠道组织形态学及豆粕饮食引起的变化评价
DOI：
10.1111/are.14361
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发表时间：
2020
影响因子：
2
通讯作者：
Wang Ling
中科院分区：
文献类型：
--
作者：
Yang Manqi;Rahimnejad Samad;Zhang Chunxiao;Song Kai;Lu Kangle;Wang Ling
开展了两项研究：(A)研究牛蛙(Lithobates)的胃肠道(GI)特性；(B)评价豆粕(SBM)完全替代鱼粉(FM)对胃肠道健康的影响。为了描述胃肠道的特征，从20只牛蛙(80克)中采集了食道、胃和肠的切片，这些牛蛙以FM为基础饮食。结果表明，牛蛙的胃肠道由粘膜、粘膜下层、肌层和浆膜四层组织层组成，肠道可分为十二指肠、空肠、回肠和直肠。此外，粘膜皱褶的丰度和长度表明，空肠是消化和吸收营养物质的主要场所。在第二项研究中，配制了一种含44%鱼粉的日粮作为鱼粉基础日粮，并用SBM完全替代鱼粉制备了SBM日粮。将60只牛蛙(35g±1g)随机分成3个重复，分别饲喂试验饲料，每天2次，连续8周。评价了随着饲料蛋白质来源的改变，胃肠道的pH值和形态的变化。结果表明，SBM组大鼠胃肠道pH值升高，但不包括贲门、空肠和直肠。此外，SBM饲料还引起了粘膜皱褶的数量、高度和宽度的显著减少，而固有层厚度的变化趋势则相反。此外，完全用SBM替代FM会导致粘膜下层和肌肉层的分离，并导致粘膜上皮损伤的发生率。本研究结果表明，空肠是营养物质消化吸收的主要部位。此外，研究还表明，完全用SBM替代FM对牛蛙的胃肠道健康产生了不利影响。
Two studies were carried out to (a) characterize the gastrointestinal (GI) tract of bullfrogRana(Lithobates)catesbeianaand (b) to evaluate the effects of total replacement of fish meal (FM) with soybean meal (SBM) on GI tract health. For characterization of the GI tract, oesophagus, stomach and intestine sections were sampled from 20 bullfrogs (80 g) fed a FM‐based diet. The results revealed that the GI tract of bullfrog is consisted of four tissue layers including mucosa, submucosa, muscularis and serosa, and that the intestine could be divided into duodenum, jejunum, ileum and rectum. Furthermore, the abundance and length of mucosal folds suggested that jejunum is the primary site of digestion and absorption of nutrients. In the second study, a diet containing 44% fish meal (FM) was formulated and used as a FM‐based diet, and a SBM‐based diet was prepared by entire substitution of FM with SBM. Sixty bullfrogs (35 ± 1 g) were divided into three replicates of the two groups and were fed the test diets to apparent satiation twice daily for 8 weeks. The changes in pH and morphology of GI tract in response to the shift in dietary protein source were evaluated. The results showed the enhancement of pH values in GI tract of the SBM group excluding cardia, jejunum and rectum. Also, SBM diet triggered significant decreases in the number, height and width of mucosal folds, while an opposite trend was observed for thickness of lamina propria. Moreover, the entire replacement of FM with SBM led to the separation of submucosa from muscularis and incidence of lesion in mucous epithelium. The findings in this study showed that jejunum is the main site of nutrients digestion and absorption. Also, it was demonstrated that entire replacement of FM with SBM adversely influenced the GI tract health in bullfrog.