Fc and C3bi receptors and the differentiation antigen BH2-Ag are randomly distributed in the plasma membrane of locomoting neutrophils.
Fc and C3bi receptors and the differentiation antigen BH2-Ag are randomly distributed in the plasma membrane of locomoting neutrophils.
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DOI：
10.1083/jcb.110.3.661
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发表时间：
1990-03
影响因子：
7.8
通讯作者：
Michl, J
中科院分区：
文献类型：
--
作者：
Pytowski, B;Maxfield, F R;Michl, J
来自几个实验室的报告表明，在运动过程中被捕获的中性粒细胞优先结合细胞前部的免疫复合物。这种不对称的结合被解释为表明吞噬受体对细胞前部的主动调节。为了进一步研究这一点，我们使用荧光图像的数字分析来确定单克隆抗体针对Fc受体、C3的C3bi片段受体和中性粒细胞特异性抗原的结合模式。我们发现这三种蛋白沿迁移中性粒细胞的长度分布几乎相同，并且它们的分布与质膜的前后分布非常接近。单克隆抗体的使用提供了一个重要的优势，因为抗受体抗体的结合不像配体的结合，它应该独立于受体亲和力的潜在变化。我们得出结论，吞噬受体在运动中性粒细胞的质膜中的前分布与迁移细胞前部膜面积的总体增加平行，并且吞噬受体的特异性易位不会发生。
Reports from several laboratories suggest that neutrophils arrested during locomotion preferentially bind immune complexes at the front of the cell. Such asymmetry of binding has been interpreted as indicating an active modulation of phagocytic receptors to the anterior of the cell. To investigate this further, we have used digital analysis of fluorescence images to determine the binding patterns of mAbs directed against the Fc receptors, the receptors for the C3bi fragment of C3, and a neutrophil-specific antigen. We found that all three proteins are distributed nearly identically along the length of migrating neutrophils, and their distribution very closely parallels the anterior to posterior distribution of the plasma membrane. The use of mAbs offered an important advantage in that the binding of antireceptor antibodies, unlike the binding of ligands, should be independent of potential changes in the affinity of the receptors. We conclude that the anterior distribution of the phagocytic receptors in the plasma membrane of locomoting neutrophils parallels the overall increase in membrane area at the front of a migrating cell and that specific translocation of phagocytic receptors does not occur.