ROLE OF CATALYTIC SUPEROXIDE FORMATION IN O2 INHIBITION OF NITROREDUCTASE
ROLE OF CATALYTIC SUPEROXIDE FORMATION IN O2 INHIBITION OF NITROREDUCTASE
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DOI：
10.1016/0006-291x(75)90163-1
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发表时间：
1975-01-01
影响因子：
3.1
通讯作者：
HOLTZMAN, JL
中科院分区：
文献类型：
--
作者：
MASON, RP;HOLTZMAN, JL
许多硝基还原酶被氧强烈抑制。硝基还原酶活性的第一个中间体硝基芳香族阴离子自由基在需氧微粒体孵育中无法检测到。尽管硝基化合物没有变化，但呋喃妥因和对硝基苯甲酸酯都大大增加了NADPH支持的氧摄取。超氧化物歧化酶部分逆转了这种催化氧消耗，表明超氧阴离子自由基是由硝基芳族阴离子自由基的快速空气氧化形成的。
Many nitroreductases are strongly inhibited by oxygen. The first intermediate of nitroreductase activity, the nitroaromatic anion free radical, cannot be detected in aerobic microsomal incubations. Even though the nitro compounds are unchanged, both nitrofurantoin and p-nitrobenzoate profoundly increase the NADPH-supported oxygen uptake. This catalytic oxygen consumption is partially reversed by superoxide dismutase, suggesting that superoxide anion free radical is being formed by the rapid air oxidation of the nitroaromatic anion radical.