PROPERTIES OF AN ASYMMETRICAL FLOW FIELD-FLOW FRACTIONATION CHANNEL HAVING ONE PERMEABLE WALL
PROPERTIES OF AN ASYMMETRICAL FLOW FIELD-FLOW FRACTIONATION CHANNEL HAVING ONE PERMEABLE WALL
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DOI：
10.1021/ac00136a016
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发表时间：
1987-05-01
影响因子：
7.4
通讯作者：
GIDDINGS, JC
中科院分区：
文献类型：
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作者：
WAHLUND, KG;GIDDINGS, JC
我们在此描述了仅具有单个可渗透壁的流场流分级通道。该壁用作积聚壁，由载体流可渗透但样品不可渗透的膜/填料层组成。相对的壁，不可渗透的流动，是由玻璃板，使通道内容物可见。在操作中，通道具有一个进入流和两个流出流，一个是渗透膜的扩散流。这种设计在技术上比传统的、更对称的设计更简单，传统的、更对称的设计中，上壁也可以渗透流体。它表明，新的设计创建纵向和横向的流速梯度，但保留，如果足够强，可以解释与传统的渠道在很大程度上相同的方式。理论的ex-kings获得的速度梯度，保留，和松弛和聚焦时间。为了说明大分子分离的潜力，本文介绍了一种蛋白质混合物的分离方法，即场流分离技术（FFF），它是场流分离技术（FFF）家族的一个分支，具有快速、高分辨率地分离大分子和胶体物质的潜力。最近总结了流动FFF的先前工作（2）。FFF分离单元通常具有平坦、薄、矩形通道的几何形状，其中样品在一端加载，然后随着载流沿通道向下输送，以在另一端离开。当迁移通过通道时，样品受到一个力（来自一个“场”）的作用，该力使样品沿通道壁之沿着一个壁（积聚壁）有差别地积聚在低速移动的载体薄层中。这构成了保留和分离的基础。
We describe here a flow field-flow fractionation channel having only a single permeable wall. This wall, serving as the accumulation wall, consists of a membrane/frlt bllayer permeable to the carrier flow but Impermeable to the sample. The opposite wall, Impermeable to flow, is made of a glass plate, which renders the channel contents visible. In operation the channel has one Incoming flowstream and two outgoing streams, one being the diffuse stream permeating the membrane. This design is technically simpler than conven-tional, more symmetrical designs where the upper wall Is also permeable to flow. It Is shown that the new design creates both longitudinal and transverse flow velocity gradients but retention, if sufficiently strong, can be Interpreted In much the same way as with conventional channels. Theoretical ex-pressions are obtained for velocity gradients, retention, and relaxation and focusing times. A fractionation of a mixture of proteins Is shown In order to Illustrate the potential for mac-romolecular separations.Flow field-flow fractionation, one of the subtechniques in the field-flow fractionation (FFF) family (1), is potentially capable of the rapid separation of a wide variety of macro-molecular and colloidal materials at high resolution. Previous work in flow FFF was recently summarized (2). The FFF separation unit has typically the geometry of a flat, thin, rectangularchannel where the sample is loaded at one end and then transported down the channel with a carrier flow to exit at the other end. While migrating through the channel, the sample is subject to a force (derived from a “field”) which causes it to accumulate differentially in theslow moving carrier laminae along one of the channelwalls (the accumulation wall). This constitutes the basis of the retention and separation.