The staphylococcal nuclease prevents biofilm formation in Staphylococcus aureus and other biofilm-forming bacteria
The staphylococcal nuclease prevents biofilm formation in Staphylococcus aureus and other biofilm-forming bacteria
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葡萄球菌核酸酶可防止金黄色葡萄球菌和其他生物膜形成细菌的生物膜形成
DOI：
10.1007/s11427-011-4195-5
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发表时间：
2011-09-01
影响因子：
9.1
通讯作者：
Du YiWu
中科院分区：
文献类型：
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作者：
Tang JunNi;Kang MingSong;Du YiWu
葡萄球菌核酸酶由nuc1基因编码，是金黄色葡萄球菌重要的毒力因子。然而，核酸酶的生理作用尚未得到充分的表征。目前的研究发现，在金黄色葡萄球菌产生核酸酶的菌株中，生物膜的发育可以被阻止；然而，当nuc1基因被敲除时，形成生物膜的能力显著增加。采用扫描电子显微镜和共聚焦扫描激光显微镜研究了nuc1基因在生物膜形成中的作用。此外，研究还发现，nuc1基因产物、葡萄球菌核酸酶和重组nuc1蛋白对铜绿假单胞菌、胸膜肺炎放线杆菌和副猪嗜血杆菌等其他生物膜形成细菌也有明显的影响。目前的研究表明葡萄球菌核酸酶的产生与生物膜发育的预防之间存在直接关系。本研究的结果强调了葡萄球菌核酸酶活性在防止金黄色葡萄球菌生物膜形成中的重要作用。他们还为葡萄球菌核酸酶在其他生物中的生物学作用提供了证据。
The staphylococcal nuclease, encoded by the nuc1 gene, is an important virulence factor of Staphylococcus aureus. However, the physiological role of the nuclease has not been fully characterized. The current study observed that biofilm development could be prevented in staphylococcal nuclease-producing strains of S. aureus; however, when the nuc1 gene was knocked out, the ability to form a biofilm significantly increased. Scanning electron and confocal scanning laser microscopy were used to evaluate the role of the nuc1 gene in biofilm formation. Moreover, the nuc1 gene product, staphylococcal nuclease, and recombinant NUC1 protein were found to have a visible effect on other biofilm-forming bacteria, such as Pseudomonas aeruginosa, Actinobacillus pleuropneumoniae, and Haemophilus parasuis. The current study showed a direct relationship between staphylococcal nuclease production and the prevention of biofilm development. The findings from this study underscore the important role of staphylococcal nuclease activity to prevent biofilm formation in S. aureus. They also provided evidence for the biological role of staphylococcal nucleases in other organisms.