Comparison of the oral bacterial flora in saliva from a healthy subject and two periodontitis patients by sequence analysis of 16S rDNA libraries
Comparison of the oral bacterial flora in saliva from a healthy subject and two periodontitis patients by sequence analysis of 16S rDNA libraries
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DOI：
10.1111/j.1348-0421.2000.tb02545.x
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发表时间：
2000-01-01
影响因子：
2.6
通讯作者：
Benno, Y
中科院分区：
文献类型：
--
作者：
Sakamoto, M;Umeda, M;Benno, Y
采用16S rDNA文库的序列分析方法（无需培养），对两名牙周炎患者和一名牙周健康者唾液中的口腔菌群进行了比较。通过聚合酶链反应（PCR）从唾液DNA中扩增16S rDNA并进行克隆。随机选取克隆进行部分测序。根据序列相似性，将克隆归类为几个簇，对应于细菌域的主要门。文库中的主要门是低G + C革兰氏阳性菌。在健康受试者的唾液样本中未发现与牙周病原菌相关的克隆序列，而在牙周炎患者的唾液样本中检测到了一些牙周病原体，如直肠弯曲杆菌、中间普氏菌、牙龈卟啉单胞菌和索氏密螺旋体。此外，还识别出了一些以前未被鉴定和未培养的微生物。这些微生物可能在牙周疾病中起一定作用。这项研究揭示了分子生物学技术在无需培养的情况下分析与牙周疾病相关的口腔菌群（包括以前未被鉴定和未培养的微生物）的一些潜力。
The oral bacterial flora in the saliva from two patients with periodontitis and from a periodontally healthy subject were compared using a sequence analysis of 16S rDNA libraries without cultivation. 16S rDNAs were amplified from salivary DNA by PCR and cloned, Randomly selected clones were partially sequenced, On the basis of sequence similarities, the clones were classified into several clusters corresponding to the major phylum of the domain Bacteria. The major phylum in the libraries was the low G+C Gram-positive bacteria. There was no clonal sequence affiliated with periodontopathic bacteria in the salivary sample from the healthy subject, while a number of periodontal pathogens such as Campylobacter rectus, Prevotella intermedia, Porphyromonas gingivalis and Treponema socranskii were detected in the salivary samples from the patients with periodontitis, In addition, a number of previously uncharacterized and uncultured microorganisms were recognized, These organisms may have some role in periodontal disease. This study reveals some potential for a molecular-biological technique to analyze the oral microflora associated with periodontal disease, including previously uncharacterized and uncultured microorganisms, without cultivation.