Structure sensitivity in third-harmonic generation microscopy
Structure sensitivity in third-harmonic generation microscopy
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DOI：
10.1364/ol.30.002134
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发表时间：
2005-08-15
期刊：
影响因子：
3.6
通讯作者：
Beaurepaire, E
中科院分区：
文献类型：
--
作者：
Débarre, D;Supatto, W;Beaurepaire, E
我们通过实验表征了三次谐波发生 (THG) 显微镜中样品结构和光束聚焦对信号水平的影响。在均匀球形样品的情况下，可以通过修改激发光束的瑞利长度来控制信号对球体尺寸的依赖性。更一般地说，激发聚焦对信号的影响取决于样品的几何形状，从而允许人们突出显示复杂系统内的某些结构。我们通过果蝇胚胎 THG 图像中基于聚焦的对比度调制来说明这一点。 (c) 2005 年美国光学学会。
We characterize experimentally the influence of sample structure and beam focusing on signal level in third-harmonic generation (THG) microscopy. In the case of a homogeneous spherical sample, the dependence of the signal on the size of the sphere can be controlled by modifying the Rayleigh length of the excitation beam. More generally, the influence of excitation focusing on the signal depends on sample geometry, allowing one to highlight certain structures within a complex system. We illustrate this point by focusing-based contrast modulation in THG images of Drosophila embryos. (c) 2005 Optical Society of America.