Antibiotic susceptibility of 33 Prevotella strains isolated from Romanian patients with abscesses in head and neck spaces
Antibiotic susceptibility of 33 Prevotella strains isolated from Romanian patients with abscesses in head and neck spaces
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DOI：
10.1016/j.anaerobe.2014.10.006
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发表时间：
2015-10-01
期刊：
影响因子：
2.3
通讯作者：
Bari, Maria
中科院分区：
文献类型：
--
作者：
Bancescu, Gabriela;Didilescu, Andreea;Bari, Maria
本研究的目的是调查从罗马尼亚一家医院头颈部脓肿患者中分离出的33株普雷沃氏菌的易感性。应用该方法测定了青霉素G、氨苄西林、阿莫西林-克拉维酸酯、甲硝唑和克林霉素的最低抑菌浓度。此外，采用显色头孢菌素圆盘法检测β -内酰胺酶活性。结果表明，11株菌株由于产生β -内酰胺酶而对青霉素G和氨苄西林均耐药。33株普雷沃特菌对其他3种抗菌药物均敏感，但只有1株布氏普雷沃特菌对青霉素G -氨苄西林耐药（MIC为32，MIC为12 mg/L），该菌株对克林霉素（MIC为256 mg/L）也表现出高耐药性。我们的数据强调了抗菌测试的必要性，包括监测口腔-颌面-面部混合厌氧感染病例的β -内酰胺酶产生，在这种情况下，除了手术引流外，还需要抗菌治疗。研究结果表明，阿莫西林-克拉维酸酯与甲硝唑一样，对所测试的普雷沃特菌菌株具有完全的活性。然而，应定期对具有临床意义的厌氧菌进行当地和多中心的耐药性调查。(C) 2014 Elsevier Ltd.版权所有。
The purpose of this study was to investigate the susceptibility of a series of 33 Prevotella strains isolated from patients with abscesses in the head and neck spaces, presented to one Romanian hospital. The Etest was applied to determine the value of the minimum inhibitory concentrations for: penicillin G, ampicillin, amoxicillin-clavulanate, metronidazole and clindamycin. In addition, the beta-lactamase activity was detected by the chromogenic cephalosporin disc method. The results indicated that 11 isolates were resistant to both penicillin G and ampicillin due to the beta-lactamase production. All the 33 Prevotella strains were susceptible to the other 3 antimicrobial agents tested, except for only one penicillin G - ampicillin resistant isolate of Prevotella buccae (MIC > 32 and MIC = 12 mg/L, respectively), which showed high resistance to clindamycin (MIC > 256 mg/L) too. Our data underline the necessity for antimicrobial testing including monitoring of beta-lactamase production in cases of oro-maxillo-facial mixed anaerobic infections where antimicrobial treatment is required in addition to the surgical drainage. The results of the study indicated that amoxicillin-clavulanate, like metronidazole, was fully active against the tested Prevotella strains. However, local and multicentre surveys on drug resistance among the clinically significant anaerobic isolates should be carried out periodically. (C) 2014 Elsevier Ltd. All rights reserved.