In vitro Screening of Traditional Chinese Medicines Compounds Derived with Anti-encephalomyocarditis Virus Activities
In vitro Screening of Traditional Chinese Medicines Compounds Derived with Anti-encephalomyocarditis Virus Activities
复制标题
抗脑心肌炎病毒活性中药化合物的体外筛选
DOI：
10.1007/s12257-019-0354-5
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发表时间：
2020-04-20
影响因子：
3.2
通讯作者：
Sun, Na
中科院分区：
文献类型：
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作者：
Zheng, Jiangang;Xu, Yinlan;Sun, Na
脑心肌炎病毒的流行给地球仪养猪业带来了巨大的经济损失。中草药具有潜在的抗病毒活性，这已被证实。对15种中药复方进行抗脑心肌炎病毒（EMCV）活性筛选。用MTT法和抗病毒试验测定了该化合物对EMCV的最大无毒浓度（MNTC）、半数细胞毒浓度（CC_（50））、最大抑制率（MIR）和半数有效浓度（EC_（50））。选择MIR为50%、选择指数（SI）为3的化合物黄芩苷和苦参碱进行进一步的病毒载量分析。结果表明，黄芩苷和苦参碱的MIRs均高于阳性对照利巴韦林，而SI值则明显小于对照。实时定量PCR分析表明，黄芩苷和苦参碱具有显着（p 0.05）的抗EMCV活性相比，对照。这是一项基线研究，结论是黄芩苷和苦参碱需要进一步开发作为治疗EMCV感染的独立药物或中药处方的一部分。
The prevalence of encephalomyocarditis virus has brought about enormous financial losses to the swine industry throughout the globe. Chinese herbal medicines have potential antiviral activity which has been proved. Fifteen traditional Chinese medicine compounds were screened for anti-encephalomyocarditis virus (EMCV) activity. The maximum non-toxic concentration (MNTC), cytotoxic concentration 50% (CC50), maximal inhibition rate (MIR), and effective concentration 50% (EC50) against EMCV were measured using MTT and antiviral assays on baby hamster Syrian kidney (BHK-21) cells. Two of the compounds, baicalin and matrine, with MIR > 50% and selective index (SI) > 3 were chosen for further virus load analysis. The results showed that the MIRs of baicalin and matrine were higher than that of positive control ribavirin while the SI values were much smaller than that of the control. Real-time quantitative PCR analysis demonstrated that baicalin and matrine have significant (p < 0.05) anti-EMCV activity compared to the control. It is a baseline study concluded that baicalin and matrine needs further development as an independent drugs or part of a Chinese medicine prescription for the treatment of EMCV infection.